We narrowed to 65,370 results for: eng

1. pWPXL-p150

   Plasmid

   #160992

   Purpose

   Express fulllength of mouse p150 protein

   Depositor

   Zhiguo Zhang

   Article

   Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

   Insert

   CHAF1A (Chaf1a Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE2-TetO-GFP-mSox2

   Plasmid

   #206374

   Purpose

   Expresses EGFP fused mouse SOX2 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSET-pr-mEos4b (mEosFP4b-V69T)

   Plasmid

   #99226

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos4b
   

   Expression

   Bacterial

   Mutation

   V69T for primed conversion applications

   Promoter

   T7

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBTK614

   Plasmid

   #110608

   Purpose

   BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIRECT_23D

   Plasmid

   #91141

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBTK601

   Plasmid

   #110607

   Purpose

   BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Cas9
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

   Plasmid

   #136381

   Purpose

   CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b (D570A)-TV-T2A-MCP-TV
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D570A

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDIRECT_25G

   Plasmid

   #91144

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDIRECT_25I

   Plasmid

   #91146

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTK-EdnrbE2-Sox10-FLAG-tev-AVI

    Plasmid

    #127775

    Purpose

    Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tag

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    sox10 (SOX10 Chicken)
    

    Use

    Enhancer reporter construct

    Tags

    Avi

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDIRECT_22D

    Plasmid

    #91136

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNTPTP alpha (S180A/S204A)

    Plasmid

    #17700

    Depositor

    Xinmin Zheng

    Article

    Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

    Insert

    PTPalpha S180A S204A (PTPRA Human)
    

    Expression

    Mammalian

    Mutation

    S180A / S204A

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pDIRECT_21D

    Plasmid

    #91131

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. NG1237 pCI-TPI-PTC160-4H

    Plasmid

    #65804

    Purpose

    NMD reporter mRNA

    Depositor

    Niels Gehring

    Article

    Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

    Insert

    TPI (TPI1 Human)
    

    Tags

    4H (4 copies of short beta-globin 3'UTR for …

    Expression

    Mammalian

    Mutation

    premature stop codon at 160

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJY-RpABE-FT_INF

    Plasmid

    #112874

    Purpose

    binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis

    Depositor

    Jin-Soo Kim, Jae-Young Yun

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA targeting FT
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. UASgal-A9-Pleum

    Plasmid

    #60343

    Purpose

    Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Expression

    Yeast

    Available Since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pMXs-1003

    Plasmid

    #31712

    Depositor

    Duanqing Pei

    Article

    Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

    Insert

    miR-367 (Mir367 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

18. CNC3 (CASANOVA-C3)

    Plasmid

    #137191

    Purpose

    Expresses CNC3 (CASANOVA-C3), a blue light dependent NmeCas9 inhibitory protein

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

    Insert

    CNC3 (CASANOVA-C3)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSET-pr-mEos4a (mEosFP4a-V69T)

    Plasmid

    #99225

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos4a (mEos4a-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos4a
    

    Expression

    Bacterial

    Mutation

    V69T for primed conversion applications

    Promoter

    T7

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PatoG-OG241

    Plasmid

    #72839

    Purpose

    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoG
    

    Expression

    Bacterial

    Promoter

    PatoG

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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