We narrowed to 10,073 results for: control

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AAVS1-Puro cTnT CCND2-T2A-iChloC

Plasmid

#179842

Purpose

donor plasmid for cardiac-specific CCND2-T2A-iChloc expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

improved chloride-conducting ChR (iChloC)

Expression

Mammalian

Promoter

Troponin T promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-luciferase

Plasmid

#179844

Purpose

donor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR, Luciferase, and TALEN ; Donor plasmid

Tags

luciferase

Expression

Mammalian

Promoter

Troponin T promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-WT

Plasmid

#218460

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues)-wild type (S )

Expression

Mammalian

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

Plasmid

#218461

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )

Expression

Mammalian

Mutation

Thr1273AGlu substitution

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

Plasmid

#218462

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )

Expression

Mammalian

Mutation

Thr1273Asp substitution

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-Aurora B KD

Plasmid

#184043

Purpose

Expresses H2B-GFP fused to Aurora B kinase dead

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

AURKB (AURKB Human)

Tags

EGFP and H2B

Expression

Mammalian

Mutation

Changed Lysine 65 to Arginine

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-RepoMan-wt

Plasmid

#184269

Purpose

Expresses shRNA resistant GFP-RepoMan

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell division cycle associated 2 (CDCA2 Human)

Tags

3xHA and GFP

Expression

Mammalian

Mutation

changed nucleotides 723-747, 1488-1509, 1524-1548…

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-S473L-pHluorin

Plasmid

#185540

Purpose

Expresses mutant S473L LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed serine 473 for leucine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-Y433A-pHluorin

Plasmid

#185541

Purpose

Expresses mutant Y433A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed tyrosine 433 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-R474Q-pHluorin

Plasmid

#185542

Purpose

Expresses mutant R474Q LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed arginine 474 for glutamine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-T380A-pHluorin

Plasmid

#185543

Purpose

Expresses mutant T380A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed threonine 380 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-E383A-pHluorin

Plasmid

#185544

Purpose

Expresses mutant E383A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed glutamic acid 383 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-C200R-pHluorin

Plasmid

#185545

Purpose

Expresses mutant C200R LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed cysteine 383 for arginine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(AAVS1-1)-PGKpuroBFP-W

Plasmid

#211954

Purpose

Express safe-cutter control gRNA with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

safe-cutter control sgRNA_AAVS1-1 (AAVS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(AAVS1-2)-PGKpuroBFP-W

Plasmid

#211955

Purpose

Express safe-cutter control gRNA with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

safe-cutter control sgRNA_AAVS1-2 (AAVS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Gata6-3xT7

Plasmid

#200889

Purpose

Inducible lentiviral expression of Gata6

Depositor

Kai Ge

Article

Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

Insert

Gata6 (Gata6 Mouse)

Use

Lentiviral; Doxycycline inducible

Tags

3xT7

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b MOSPD2 [315-445]

Plasmid

#186474

Purpose

Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [315-445]

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-chGrb2/eDHFR(69K6)-iRFP713

Plasmid

#178854

Purpose

Mammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713 (GRB2 Human)

Expression

Mammalian

Mutation

Grb2: Y160E

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s

Plasmid

#172105

Purpose

Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)-Gαs (GNAS Human)

Expression

Mammalian

Mutation

Gαs: C3S

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat(G1A)-mCherry

Plasmid

#181852

Purpose

mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Tags

mCherry

Expression

Mammalian

Mutation

Glycine residue immediately after start site in P…

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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