We narrowed to 17,000 results for: puromycin

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pEBTet-β-tubulin_SNAP

Plasmid

#136831

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCW-IRF4-Puro

Plasmid

#123323

Purpose

Lentiviral vector for expression of IRF4 under a Doxycycline inducible promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

IRF4 (IRF4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

several silent mutations to disrupt sgRNA targeti…

Promoter

Doxycycline inducible minimal CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-WT1shRNA

Plasmid

#220560

Purpose

To inducibly knockdown WT1 or EWSR1::WT1 expression

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

WT1 shRNA (WT1 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMXsIP GFP-VPS33A

Plasmid

#67022

Purpose

human VPS33A ORF was inserted into pMXsIP SPGFP-GS Ci2 vector

Depositor

Noboru Mizushima

Article

Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

Insert

Vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) (VPS33A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
pSLQ1371-sgSTAT3-1

Plasmid

#121425

Purpose

sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgSTAT3-1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMMLV-EGFP-P2A-Puro

Plasmid

#160226

Purpose

Expresses EGFP in mammalian cells

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

EGFP-P2A-PuroR

Use

Retroviral

Expression

Bacterial and Mammalian

Promoter

LTR

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-IMS-RP

Plasmid

#24535

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

Diablo homolog (DIABLO Human)

Use

Retroviral

Tags

mRFP1

Expression

Mammalian

Mutation

Deleted residues 56-239

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro GFP shRNA

Plasmid

#10675

Purpose

Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

Depositor

William Hahn

Article

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

GFP shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-TBK1-gRNA (PX459)

Plasmid

#221550

Purpose

Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human TBK1 (TBK1 Human)

Expression

Mammalian

Promoter

u6

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEBTetD-β-tubulin_SNAP

Plasmid

#136832

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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EF13E

Plasmid

#42942

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

EN- Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

AgeI-BclI fragment swapped with nt 1927-3708 from…

Promoter

CMV MIE, L1 5'UTR

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-HsSAS6

Plasmid

#136789

Purpose

Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-SAS6 (SASS6 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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MelanoTag-Control-PURO (pLJC5-GPR143-mScarlet-1xMyc)

Plasmid

#160368

Purpose

Stable expression of GPR143-mScarlet-1xMyc fusion protein for use as a control with MelanoTag-PURO

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

GPR143 (GPR143 Human)

Use

Lentiviral

Tags

mScarlet-myc

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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RT3GEPIR

Plasmid

#111169

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Retroviral

Mutation

WT

Promoter

T3G

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-Pur-CAG-hM3Dq-mCherry

Plasmid

#80948

Purpose

human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

Insert

hM3Dq-mCherry

Tags

mcherry fusion

Expression

Mammalian

Promoter

CAG promoter

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-Pur-CAG-hM4Di-mCherry

Plasmid

#80947

Purpose

human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

Insert

hM4Di-mCherry

Tags

mcherry fusion

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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mTAGBFP2 WC visual barcode

Plasmid

#158666

Purpose

tagging clonal populations with whole cell mTAGBFP2 fluorescent protein

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_MYRF

Plasmid

#64843

Purpose

A lentiviral vector for the expression of mouse MYRF under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

MYRF (Myrf Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-AtAFB2

Plasmid

#129715

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

no

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG J591-scFV T-CAR

Plasmid

#171961

Purpose

Donor plasmid for J591 T-CAR expression in human cells

Depositor

Xiaoping Bao

Article

Harris et al J Immunol Regen Med. 2023 May;20:100074.

Insert

Synthetic J591-scFV CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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