We narrowed to 10,128 results for: Met
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Plasmid#110219PurposeExpression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
TagsHis6 and ZBExpressionBacterialMutationY117C, P296L (see depositor comments below)Available sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-3F2
Plasmid#73428PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.DepositorInsertRepressor C4 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-5F5-mCherry
Plasmid#73422PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.DepositorInsertPromoter 5F5 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP5F5 (orthogonal T7-lac variant)Available sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT mir-1-1 reporter cel-mir-40
Plasmid#46739DepositorInsertcel-mir-40
UseRNAiTagsHis and hsa-mir-1-1ExpressionInsectPromoterMetallothionein (MT)Available sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R61E
Plasmid#60353PurposeR61E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
TagsHis and TEV protease siteExpressionBacterialMutationR61E; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#48259PurposeTemplate plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
Ag TEF1 promoter
UseRoutine cloning vectorMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterAg TEF1 promoterAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
GRM7
Plasmid#28110PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGRM7:DCC014-D04:C211307 (GRM7 Human)
TagsHis tag with TEV siteExpressionBacterialMutationcontains ligand binding domain, residues 37-513.Available sinceMarch 18, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMD-MsePCNA3
Plasmid#107075PurposeM. sedula PCNA3DepositorInsertM. sedula PCNA3
UseCloning vectorPromoterSP6, lacAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMD-MsePCNA2
Plasmid#107074PurposeM. sedula PCNA2DepositorInsertM. sedula PCNA2
UseCloning vectorPromoterSP6, lacAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMD-MsePCNA1
Plasmid#107073PurposeM. sedula PCNA1DepositorInsertM. sedula PCNA1
UseCloning vectorPromoterSP6, lacAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTH_818
Plasmid#255509PurposeDERA enzyme production under control of T7 promoter and inducible lac operatorDepositorInsert2-deoxyribose-5-phosphate aldolase (DERA) from Methanothermobacter thermautotrophicus
Tags6xHis tag-TEV protease recognition site (ENLYFQ/G…ExpressionBacterialMutationWTPromoterT7Available sinceMay 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K
Plasmid#112652PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K
ExpressionYeastMutationR59K (GAP-dead)PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pM2P20X
Plasmid#108210PurposeExpresses MSePCNA2-PdX fusion protein in E. coliDepositorInsertMsePCNA1-PdX fusion protein
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM3C
Plasmid#108212PurposeExpresses MsePCNA3-CYP101A1 fusion protein in E. coliDepositorInsertMsePCNA3-CYP101A1 fusion protein
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM1R
Plasmid#108208PurposeExpresses MsePCNA1-PdR fusion protein in E. coliDepositorInsertMsePCNA1-PdR fusion protein
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15T-MsePCNA2
Plasmid#107064PurposeExpresses M. sedula PCNA2 in E. coliDepositorInsertM. sedula PCNA2
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15T-MsePCNA1
Plasmid#107063PurposeExpresses M. sedula PCNA1 in E. coliDepositorInsertM. sedula PCNA1
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15T-MsePCNA3
Plasmid#107065PurposeExpresses M. sedula PCNA3 in E. coliDepositorInsertM. sedula PCNA3
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable sinceFeb. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
18065-M03-412
Plasmid#225666PurposeLentivirus protein expression. Control plasmid.DepositorInsertsUseLentiviralTags3xFLAGMutationC-terminally truncated (aa 1-333 only)PromoterWPRE-SV40p and mCMVpAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 2xHA GPR137
Plasmid#226813PurposeTet-inducible lentiviral vector for 2xHA GPR137 expressionDepositorInsertGPR137 (GPR137 Human)
UseLentiviralTags2xHA tag followed by a GDPPVAT linkerMutationSequence optimized from NP_001164351Available sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only