We narrowed to 81,618 results for: Mycs;

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pEK23

Plasmid

#210595

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdH4pro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

BdH4pro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEK24

Plasmid

#210597

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdACTINpro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

BdACTINpro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP12-talinC11-ABS3mut-mEmerald

Plasmid

#202383

Purpose

FKBP12-Talin(1974-2541)(K2443D)(V2444D)(K2445D)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

FKBP12-Talin(1974-2541)(K2443D)(V2444D)(K2445D)-mEmerald

Expression

Mammalian

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pYUBcLIC-GFP

Plasmid

#183509

Purpose

The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.

Depositor

Martin Högbom

Article

Grave et al Protein Expr Purif. 2022 Oct;198:106132.

Type

Empty backbone

Tags

10x His-tag and folding reporter GFP

Expression

Bacterial

Promoter

T7 lac

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pTE1069

Plasmid

#186629

Purpose

SCB-GFP E. coli reporter plasmid. Encodes repressor ScbR and ScbAp promoter upstream of GFP.

Depositor

Eriko Takano

Article

Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

Inserts

scbR
scbAp promoter

Use

Synthetic Biology

Tags

6xArg

Expression

Bacterial

Promoter

BBa_J23100 and scbAp

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9339 (pgRNA_VII-1_NatMX)

Plasmid

#161591

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9340 (pgRNA_VIII-1_NatMX)

Plasmid

#161592

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9342 (pgRNA_XIII-1_NatMX)

Plasmid

#161594

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9344 (pgRNA_XVI-1_NatMX)

Plasmid

#161596

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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KBB438

Plasmid

#185092

Purpose

GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Expression

Yeast

Mutation

Rfa1 with amino acids 338-343 deleted

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB372

Plasmid

#185084

Purpose

GAL::Rfa1[330-621]-GFP under control of GAL1 promoter

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Tags

GFP

Expression

Yeast

Mutation

Rfa1 amino acids 330-621

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pNB201

Plasmid

#169112

Purpose

TESEC expression vector for Alr from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

alr (alr Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB203

Plasmid

#169114

Purpose

TESEC expression vector for mEGFP-Alr fusion construct

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

mEGFP-alr (alr Mycobacterium tuberculosis H37Rv, Synthetic)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB204

Plasmid

#169115

Purpose

TESEC expression vector for 6xHis-Alr fusion constrcut

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

6xHis-alr (alr Mycobacterium tuberculosis H37Rv, Synthetic)

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB205

Plasmid

#169116

Purpose

TESEC expression vector for Asd from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

asd (asd Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB206

Plasmid

#169117

Purpose

TESEC expression vector for CysH from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

cysH (cysH Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB207

Plasmid

#169118

Purpose

TESEC expression vector for DapB from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

dapB (dapB Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB208

Plasmid

#169119

Purpose

TESEC expression vector for TrpD from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

trpD (trpD Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR

Plasmid

#169499

Purpose

Tier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven YPet-P2aA-PuroR

Expression

Mammalian

Promoter

PRBSA

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1047-Tier1-PhCMV-YPet-p2A-PuroR

Plasmid

#169498

Purpose

Tier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven YPet-P2aA-PuroR

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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