We narrowed to 8,487 results for: Set;

1. pAAV-U6-gRNA-CBh-mCherry

   Plasmid

   #91947

   Purpose

   Expression of empty gRNA cassette and mCherry

   Depositor

   Jimok Kim

   Article

   Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBTK622

   Plasmid

   #180592

   Purpose

   tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

   Depositor

   Jeffrey Barrick

   Article

   Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

   Inserts

   tdk
   kanR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO-MCS

   Plasmid

   #185594

   Purpose

   Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-CA

   Plasmid

   #20960

   Purpose

   piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   Gateway Destination Vector
   

   Use

   piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-roxSTOProx-ZsGreen

   Plasmid

   #51274

   Purpose

   Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   roxSTOProx-ZsGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDZ416 (24PP7SL loxP-Kan-loxP)

   Plasmid

   #45163

   Depositor

   Daniel Zenklusen, Robert Singer

   Article

   Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

   Insert

   24xPP7 Stem Loop Cassette
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCXLE-gw

   Plasmid

   #37626

   Purpose

   Non-integrating (episomal) expression vector with Gateway cassette

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2X_pX458_pSpCas9(BB)-2A-GFP

   Plasmid

   #172221

   Purpose

   Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC575

   Plasmid

   #127976

   Purpose

   AMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   ble (bleomycin resistance marker)
   

   Use

   Fungal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans trpC promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-DRGFP

    Plasmid

    #113193

    Purpose

    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

    Depositor

    Adam Karpf

    Insert

    DR-GFP
    

    Use

    Donor construct with t2a-hygromycin for gene trap…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVTHM

    Plasmid

    #12247

    Purpose

    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid

    Depositor

    Didier Trono

    Article

    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSP618

    Plasmid

    #169235

    Purpose

    Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

    Depositor

    William Greenleaf

    Article

    Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

    Insert

    mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACYCDuet-1_Ec_rpoZ

    Plasmid

    #128837

    Purpose

    E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

    Depositor

    Seth Darst

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Insert

    rpoZ (rpoZ E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRel

    Plasmid

    #227581

    Purpose

    Release plasmid with Cas9 working cassette, apramycin resistance, and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSEED-mGreenLantern:ALFA

    Plasmid

    #219644

    Purpose

    Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mGreenLantern:ALFA SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEED-sfGFP

    Plasmid

    #219639

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNBU2_erm-TetR-P1T_DP-GH023

    Plasmid

    #90324

    Purpose

    Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG

    Plasmid

    #85225

    Purpose

    Expresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassette

    Depositor

    John Naughton

    Inserts

    Optimized G Protein
    Enhanced GFP
    TVA (CD320 Chicken)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6a-kanMX6

    Plasmid

    #39296

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-CMV-polysite

    Plasmid

    #122843

    Purpose

    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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