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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,677 results for: cat

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Showing: 5861 - 5880 of 19677 results

  1. Pdpy-30-miniCAFE

    Plasmid
    #170115
    Purpose
    Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.
    Depositor
    Zhili Rong
    Publication
    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MiniCAFE
    Use
    CRISPR
    Mutation
    D8A, HNH-truncation (Δ495–609 aa) in CjCas9
    Promoter
    Dpy-30
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170115-plasmid-map-sequence-id-336055

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  2. pOPINB-HOIL-1 full-length (1–510)

    Plasmid
    #193858
    Purpose
    Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli
    Depositor
    Bernhard Lechtenberg
    Publication
    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:
    Insert
    HOIL-1 (RBCK1 Human)
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    Codon optimised for expression in E. coli
    Promoter
    T7
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    193858-plasmid-map-sequence-id-379617

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  3. S10A mutant of H3S10ph biosensor

    Plasmid
    #120810
    Purpose
    FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Publication
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
    Expression
    Mammalian
    Mutation
    Serine 10 is mutated to Alanine, and Threonine 3,…
    Promoter
    CMV
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120810-plasmid-map-sequence-id-235276

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  4. AAV-KrasWT/sgKras/Cre

    Plasmid
    #99848
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Publication
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99848-plasmid-map-sequence-id-193786

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  5. HA-hDAGLa-V5

    Plasmid
    #87674
    Purpose
    Expresses human DAGLa protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal
    Depositor
    Pat Doherty
    Publication
    Zhou et al Mol Cell Neurosci. 2016 Oct;76:76-86. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human Diacylglycerol Lipase Alpha (DAGLA Human)
    Tags
    HA and V5 tag
    Expression
    Mammalian
    Mutation
    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…
    Promoter
    T7
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87674-plasmid-map-sequence-id-173603

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  8. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Publication
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

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  9. HS_NAM-B1

    Plasmid
    #154064
    Purpose
    Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT
    Depositor
    Cristobal Uauy
    Publication
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    Hygromycin phosphotransferase
    Cre recombinase
    LoxP-flanked GUS and TtNAM-B1
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Expression
    Bacterial and Plant
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    HvHSP17, OsAct, and ZmUbi
    Available since
    Sept. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154064-plasmid-map-sequence-id-313309

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  12. pJK01_Rho_minprox_DsREd

    Plasmid
    #173489
    Purpose
    PCR template for reporter gene with mouse Rhodopsin promoter with DsRed
    Depositor
    Barak Cohen
    Publication
    White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mouse Rhodopsin promoter driving DsRed reporter gene
    Expression
    Mammalian
    Promoter
    Mouse Rhodopsin
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    173489-plasmid-map-sequence-id-339547

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  19. pGDP1 fosA

    Plasmid
    #112889
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Publication
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    fosA
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112889-plasmid-map-sequence-id-219899

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Showing: 5861 - 5880 of 19677 results