We narrowed to 18,457 results for: multi

1. pHSP70B-CP6-pac

   Plasmid

   #165877

   Purpose

   Basic Puromycin resistance cassette in Alpha1, lacks physical marker or attB site

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Basic Selection Vector
   

   Use

   Synthetic Biology

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ-dpcoCas9-EV2.1

   Plasmid

   #158411

   Purpose

   CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHSP70B-CP6-TK

   Plasmid

   #165881

   Purpose

   Basic ganciclovir or acyclovir sensitvity cassette in Alpha2, lacks physical marker or attB site

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Basic Selection Vector
   

   Use

   Synthetic Biology

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. VV201: ChR2(C128S)-eGFP in fubi

   Plasmid

   #58510

   Purpose

   Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   ChR2(C128S)-eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Cysteine 128 to Serine

   Promoter

   Ubiquitin

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3XP3-WhiteA2

   Plasmid

   #165875

   Purpose

   Basic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB site

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Eye Marker Cassette
   

   Use

   Synthetic Biology

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEU3-NII-FLAGLICNot

   Plasmid

   #140184

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, FLAG-tag and NotI restriction site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   FLAG

   Promoter

   T7

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CeNL(Ca2+)_1.2μ-pcDNA3

   Plasmid

   #111926

   Purpose

   Cyan color bright luminescent calcium indicator with intermediate affinity

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_1.2μ
   

   Expression

   Mammalian

   Mutation

   E67D, E104D, D133E at CaM

   Promoter

   CMV

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p33Fn

   Plasmid

   #158155

   Purpose

   Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

   Depositor

   Kabin Xie

   Article

   Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Mutation

   Rice codon optimized

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMZ-432

   Plasmid

   #195463

   Purpose

   Lentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: ZF3-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pSFFV - SMASh - ZF3 - p65 - ERT2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMZ-431

    Plasmid

    #195462

    Purpose

    Lentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: ZF3-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pSFFV - ZF3 - p65 - ERT2 - SMASh
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-NES-MBP-cATOM(Ras)

    Plasmid

    #209711

    Purpose

    Mammalian expression of c-ATOM biosensor for HRas with nuclear export signal

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    NES-MBP-cATOM(Ras)
    

    Tags

    NES-MBP

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti_CMV:Flag-CeNLuc_SV40:PuroR

    Plasmid

    #183036

    Purpose

    Expresses the FLAG-tagged CeNLuc (Blue LumiFluor) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    CeNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2 GFP-GSK3-MAPK-E3mut

    Plasmid

    #29691

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    GFP glycogen synthase kinase 3 recognition site E3 ligase site mutated primed by MAPK
    

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    recognition sites only, and E3 ligase site mutated

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

14. OeNL(Ca2+)_18μ-pcDNA3

    Plasmid

    #111928

    Purpose

    Orange color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    OeNL(Ca2+)_18μ
    

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR221-Firefly

    Plasmid

    #79495

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Firefly Luciferase CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    Firefly Luciferase
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-sgRbm10/Cre

    Plasmid

    #89648

    Purpose

    Expresses an Rbm10-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Rbm10 (Rbm10 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6_(GLuc)_TOP1

    Plasmid

    #68423

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Salsa-BC82-P1G10

    Plasmid

    #202923

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC82

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC82
    

    Expression

    Mammalian

    Promoter

    Tet Response Element (TRE)

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLOT-syb-sp-YFP1-10

    Plasmid

    #73700

    Purpose

    Neuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASP

    Depositor

    Marco Gallio

    Article

    Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

    Insert

    Neuronal-synaptobrevin:spYFP1–10 chimera
    

    Use

    Lexaop reporter vector

    Expression

    Insect

    Available Since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. psiCHECK-2-Zfp36-3'UTR

    Plasmid

    #61780

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Zfp36

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Zfp36 3'UTR
    

    Use

    Luciferase

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only