161,473 results

1. pCB-1

   Plasmid

   #210387

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEP PolI

   Plasmid

   #11722

   Depositor

   Lawrence Loeb

   Article

   Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

   Insert

   DNA polymerase I EP (polA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D424A, I709N, A759R. Error-prone version of PolI.

   Promoter

   Available since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. nls-EGFP

   Plasmid

   #67652

   Purpose

   Mammalian expression vector driving nls-EGFP.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   nls-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP/golgin-160

   Plasmid

   #180630

   Purpose

   Expression of WT golgin-160

   Depositor

   Carolyn Machamer

   Article

   Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

   Insert

   golgin-160 (GOLGA3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (Lentiviral Prep)

   Viral Prep

   #92379-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .

   Depositor

   John Doench, David Root

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Promoter

   Tags

   None

   Available since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

6. lenti-mAmetrine-CAAX

   Plasmid

   #216132

   Purpose

   Lentiviral vector for expressing mAmetrine localizing to the plasma membrane.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-mAmetrine-CAAX-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

   Plasmid

   #83842

   Purpose

   Fluorescent probe for S/G2 cell cycle transition and G2/M transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP12385 - pAAV-AiE0548h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2385)

   Plasmid

   #223990

   Purpose

   AiE0548h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CD63-pEGFP C2

   Plasmid

   #62964

   Purpose

   full length human CD63 cloned into pEGFP C2

   Depositor

   Paul Luzio

   Insert

   CD63 molecule (CD63 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CD63 has 1 silent mutation compared to the coding…

   Promoter

   CMV

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1

    Plasmid

    #58435

    Purpose

    Induces Citrine expression from a promoter containing 8 lexA boxes in yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    CitrineA206K
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    insul-(lexA-box)8-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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