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p221k-erTq2CFP

Plasmid

#71264

Purpose

Entry clone containing ER-localized Turqoise2. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system.

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

Tq2CFP

Use

Gateway

Tags

ER retention signal HDEL and signal peptide (ER)…

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ2452 - [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159854

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-154

Plasmid

#118536

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mOrange2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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DNMT3b KO Neo

Plasmid

#16651

Depositor

Bert Vogelstein

Article

Rhee et al Nature. 2002 Apr 4. 416(6880):552-6.

Insert

DNMT3b knock-out construct (DNMT3B Human)

Expression

Bacterial

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pMRE-Tn7-131

Plasmid

#118549

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-BE-YE

Plasmid

#107686

Purpose

Expresses dLbCpf1-BE-YE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE-YE

Expression

Mammalian

Mutation

APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-136

Plasmid

#118522

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-myc3-FBW5 delta F

Plasmid

#19912

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

F-box and WD repeat domain containing 5 delta F (FBXW5 Human)

Tags

myc3

Expression

Mammalian

Mutation

deleted aa1-42 (F-box)

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pBT3024

Plasmid

#122542

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_mt::CemOrange2

Tags

MLSLRQSIRFFKPATRTLCSSRTLL

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-141

Plasmid

#118525

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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gRNA_DNMT3b

Plasmid

#41823

Purpose

Expresses a guide RNA (gRNA) to target DNMT3b for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_DNMT3b (DNMT3B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only
pFRT/TO/HIS/FLAG/HA-PES1

Plasmid

#38085

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

PES1 pescadillo ribosomal biogenesis factor 1 (PES1 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RVQ

Plasmid

#200187

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editing

Depositor

Yiping Qi

Article

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-RVQ

Use

CRISPR; Gateway compatible lbcas12a-rv entry clone

Tags

NLS

Expression

Plant

Mutation

G146R, R182V, E795Q

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRSpuro shID1#6

Plasmid

#19165

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 1 (ID1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pNH13 (pmyo-2::QuasAr::mOrange)

Plasmid

#130273

Purpose

Expresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

QuasAr::mOrange

Tags

mOrange

Expression

Worm

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

Plasmid

#79614

Purpose

Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Inserts

SpCas9
PmCDA1

Tags

SH3 domain and SHL

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1 and pGal10

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pC012 LwCas13a locus with nontargeting spacer

Plasmid

#91904

Purpose

LwCas13a locus into pACYC184 with nontargeting spacer

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a with non-targeting spacer

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159815

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Golgi-7

Plasmid

#55420

Purpose

Localization: GalT Targeting Sequence, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Golgi (B4GALT1 Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
mTagRFP-T-Actin-7

Plasmid

#58001

Purpose

Localization: Actin, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Actin (ACTB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits