We narrowed to 10,437 results for: iCre

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CcCDP_p15A

Plasmid

#179272

Purpose

Expresses CcCDP in E. coli BL21(DE3)

Depositor

Bernd Nidetzky

Article

Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

Insert

cellodextrin phosphorylase

Use

Synthetic Biology

Tags

6xHIS

Expression

Bacterial

Promoter

T7 lacO

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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Mito-CoilE-mEm

Plasmid

#181994

Purpose

CoilE genetically encoded tag on Mito-mEmerald, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

Mito-CoilE-mEm

Tags

mEmerald

Expression

Mammalian

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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TOMM20-CoilE-mCherry

Plasmid

#181995

Purpose

CoilE genetically encoded tag on TOMM2--mCherry, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

TOMM20-CoilE-mCherry

Tags

mCherry

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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CAG.INBox-mCherry-INBox-eDHFR

Plasmid

#176203

Purpose

Double INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

INBox-mCherry-INBox-eDHFR

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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CAG.mCherry-INBox-eDHFR

Plasmid

#176202

Purpose

Single INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-INBox-eDHFR

Expression

Mammalian

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CAG.mCherry-INBox(AAA)-eDHFR

Plasmid

#176207

Purpose

INBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-INBoxAAA-eDHFR

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNArodA

Plasmid

#149658

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK129-stm2585-gp130

Plasmid

#174404

Purpose

Bacterial expression of sarA-gp130 chimera under native promoter.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA-IL6ST chimera

Expression

Bacterial

Promoter

Endogenous

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-DiB1

Plasmid

#168800

Purpose

Fluorogen activating protein (FAP) DiB1

Depositor

Alexander Mishin

Article

Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

Insert

Fluorogen activating protein DiB1

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-DiB3

Plasmid

#168802

Purpose

Fluorogen activating protein (FAP) DiB3

Depositor

Alexander Mishin

Article

Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

Insert

Fluorogen activating protein DiB3

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9H(-10TC)_arr2

Plasmid

#149587

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNAflaB

Plasmid

#149561

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD

Plasmid

#162811

Purpose

Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_(C1A)-Intein_DD

Use

Piggybac

Expression

Mammalian

Mutation

Intein(Cys1,Ala)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_M

Plasmid

#162815

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_M

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_M

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_M(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

Plasmid

#162816

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_9b

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_9b(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

Plasmid

#162817

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF6

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF6(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

Plasmid

#162818

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF7b

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF7b(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

Plasmid

#162819

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF10

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF10(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

Plasmid

#162820

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF9c/14

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF9c/14(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

Plasmid

#162821

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF3b_alt

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF3b_alt(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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