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We narrowed to 6,562 results for: nls

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Showing: 5881 - 5900 of 6562 results

  1. 6xHis-MBP-huMlCas12a

    Plasmid
    #174678
    Purpose
    Expresses 6xHis-MBP tagged MlCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huMlCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    174678-plasmid-map-sequence-id-346177

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  3. pTone-gata2aECE-nsGFP

    Plasmid
    #132975
    Purpose
    gata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backbone
    Depositor
    Nathan Lawson
    Publication
    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d
    Inserts
    nuclear localized sfGFP
    gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
    Use
    Unspecified; Transposon-mediated
    Tags
    6x myc and SV40 NLS
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    132975-plasmid-map-sequence-id-264810

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  10. pAvA141

    Plasmid
    #248146
    Purpose
    mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutations
    Depositor
    Emil Jensen
    Publication
    van Aalst et al Commun Biol. 2025 Nov 27. doi: 10.1038/s
    Insert
    luxR (gen2 mutations)
    Use
    Integration vector
    Tags
    Gal4 activation domain and nuclear localization s…
    Expression
    Yeast
    Mutation
    Gal4_AD: G8V. NLS: K120N. LuxR: N86K, K104E, M135V
    Promoter
    pPGK1
    Available since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248146-plasmid-map-sequence-id-497175

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  11. (829) SB Tet-ON dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195622
    Purpose
    Sleeping Beauty Tet-ON plasmid for induced expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    Tight TRE promoter; Tet-ON (doxycycline)
    Available since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195622-plasmid-map-sequence-id-385115

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  15. HypaR-SpCas9

    Plasmid
    #126757
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    Hypa2SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, N692A, M694A, Q695A, H698A
    Promoter
    CBh
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    126757-plasmid-map-sequence-id-412372

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  20. B-HypaSpCas9

    Plasmid
    #126763
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A; M694A; Q695A; H698A; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126763-plasmid-map-sequence-id-410968

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Showing: 5881 - 5900 of 6562 results