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pAAV-CAG-DIO-VARNAM2-Kv2.1PR

Plasmid

#195533

Purpose

Cre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP2

Plasmid

#129500

Purpose

Expresses AausFP2 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP2

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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MTK2_023

Plasmid

#123715

Purpose

Encodes pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

Plasmid

#86421

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-KAR2SS-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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mNG-Mfn1

Plasmid

#195162

Purpose

mammalian expression of Mfn1 tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

Mfn1 (MFN1 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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V5-TurboID-Mfn1

Plasmid

#195155

Purpose

mammalian expression of TurboID tagged Mfn1

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

TurboID

Tags

Mfn1

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

CMV

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.Lamp1-GFP

Plasmid

#180250

Purpose

Mammalian expression of LAMP1

Depositor

Marvin Bentley

Article

Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

Insert

LAMP1 (LAMP1 Human)

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pNXRVb-HACre

Plasmid

#78401

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
hygMX6

Expression

Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pB.IRES-Venus

Plasmid

#22666

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

B.IRES-Venus

Expression

Mammalian

Mutation

B.IRES is BICEP IRES from Sigma

Available Since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

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MSCV-JAK3(M511)_IRES_GFP

Plasmid

#206290

Purpose

Expression of mutant JAK3 (M511I) that is able to drive constitutive activation of the JAK/STAT pathway

Depositor

Charles de Bock

Article

Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

Insert

JAK3 (M511I)

Use

Retroviral

Expression

Mammalian

Mutation

Methionine 511 to Isoleucine

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3_HA_Csx30

Plasmid

#197610

Purpose

Expresses a HA-tagged version of Csx30

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

HA-tagged Csx30

Expression

Mammalian

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET-S9

Plasmid

#132948

Purpose

Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S9 (rpsI E.coli)

Tags

N-terminal 6xHis-tag

Expression

Bacterial

Mutation

E52C

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-SENP3-C526A

Plasmid

#115522

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

Insert

SENP3-C526A (Senp3 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

C526A

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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psiCheck-2-3 x mir-34

Plasmid

#40831

Purpose

Luciferase reporter

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

three sites partially complementary to miR-34 (mir-34 Fly)

Use

RNAi

Tags

luciferase

Expression

Insect

Promoter

SV40

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only
pAFM-NbrWT

Plasmid

#40830

Purpose

Expresses Flag, Myc tagged Nbr in Drosophila cells

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr (Nbr Fly)

Tags

3xFLAG and 6xMyc

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pYHY1

Plasmid

#215926

Purpose

E. coli-Bacteroides shuttle vector.

Depositor

Shannon Sirk

Article

Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

Type

Empty backbone

Use

Synthetic Biology; E. coli-bacteroides shuttle ve…

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mSynCAM 4

Plasmid

#115499

Purpose

expression of mouse full-length SynCAM 4

Depositor

Thomas Biederer

Article

Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

Insert

Cadm4 (Cadm4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-G9-5FLAG-kanMX6

Plasmid

#49181

Purpose

genomic G9-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

G9-5FLAG

Expression

Yeast

Available Since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pSicoR (GFP) cRel shRNA1

Plasmid

#22509

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

cRel shRNA1 (Rel Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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MEIS1A-pSP65

Plasmid

#21014

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1A (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only