We narrowed to 13,392 results for: mpl

1. pAG32-3HA-3FLAG

   Plasmid

   #154081

   Purpose

   For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

   Depositor

   Cornelia Kilchert

   Article

   Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

   Insert

   3xHA-3xFLAG-hygR
   

   Use

   Pcr-based tagging in yeast

   Tags

   3xHA-3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-G2-ATP8-V5 (puromycin)

   Plasmid

   #86852

   Purpose

   mammalian expression of ATP8 with G2 mitochondrial targeting sequence and V5 tag

   Depositor

   Matthew O'Connor

   Article

   Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

   Insert

   ATP8 (ATP8 Human)
   

   Use

   Tags

   ATP5G2 (G2) mitochondrial targeting signal and V5

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

   Plasmid

   #159814

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT/TO-N-mEGFP-PARP6

   Plasmid

   #178005

   Purpose

   Expresses N-terminally mEGFP-tagged PARP6

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP6 (PARP6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT/TO-N-mEGFP-PARP15

   Plasmid

   #178000

   Purpose

   Expresses N-terminally mEGFP-tagged PARP15

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP15 (PARP15 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPLINTR mCHERRY v1 backbone

   Plasmid

   #179778

   Purpose

   SPLINTR backbone vector

   Depositor

   Mark Dawson

   Article

   Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-Solo-mStayGold-PXN

   Plasmid

   #227316

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PXN Homology Arms flanking a mStayGold Tag (PXN Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. TERTpWT_Gluc_CCR5

   Plasmid

   #192270

   Purpose

   Donor template for insertion of hTERT promoter driven Gaussia luciferase reporter

   Depositor

   Shantanu Chowdhury

   Article

   Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

   Insert

   Promoter Reporter Insert within donor template
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TERT promoter driving expression of gaussia

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAd-Track Flag-SIRT1 H355A

   Plasmid

   #8439

   Depositor

   Pere Puigserver

   Article

   Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Histidine 355 mutated to Alanine

   Promoter

   Available since

   Dec. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TDP-43_CTD_A321G

    Plasmid

    #98670

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321G

    Depositor

    Nicolas Fawzi

    Article

    Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A321G

    Promoter

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-V0D1

    Plasmid

    #210348

    Purpose

    Expresses ATP6V0D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    V-ATPase V0D1 (ATP6V0D1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS114 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199367

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-SUMOEu-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. GIT1A-FLAG

    Plasmid

    #15225

    Depositor

    Rick Horwitz

    Article

    Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

    Insert

    G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-4T-1 UBXN2A

    Plasmid

    #113505

    Purpose

    Bacterial expression of GST-tagged UBXN2A

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    UBXN2A (UBXN2A Human)
    

    Use

    Tags

    GST-Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

    Plasmid

    #115789

    Purpose

    Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    NDP (NDP Human)
    

    Use

    Tags

    alkaline phosphatase and flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2T-CAG-KKHSaCas9-BlastR

    Plasmid

    #107189

    Purpose

    Confers constitutive expression of KKH SaCas9.

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Insert

    KKH SaCas9 (NEWENTRY )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E782K/N968K/R1015H

    Promoter

    Chicken ß-Actin

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-FIH1

    Plasmid

    #21399

    Depositor

    Eric Metzen

    Article

    Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

    Insert

    HIF1AN (HIF1AN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pRK5-HA-YFP-DEPTOR

    Plasmid

    #73390

    Purpose

    Expresses HA and YFP tagged DEPTOR

    Depositor

    Taekjip Ha, Jie Chen

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    DEP Domain Containing MTOR-Interacting Protein (DEPTOR Human)
    

    Use

    Tags

    HA and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pTAMAHISTEV_PfeA

    Plasmid

    #128943

    Purpose

    expresses PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75)

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-5X1 Flag hDPY30

    Plasmid

    #15556

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    DPY30 (DPY30 Human)
    

    Use

    Tags

    Flag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only