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pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)

Plasmid

#185701

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(FCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)

Plasmid

#185705

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(PDCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)

Plasmid

#185703

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(SCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)

Plasmid

#185704

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(PCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)

Plasmid

#185702

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(BCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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sgEML4

Plasmid

#85568

Purpose

Expresses sgRNA targeting human EML4 between exon 13 and 14

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

echinoderm microtubule associated protein like 4 (EML4 Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pBABE-hygro-hTERT

Plasmid

#1773

Purpose

Retroviral expression of hTERT. Used to create immortalized cell lines.

Depositor

Bob Weinberg

Article

Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

Insert

hTERT (TERT Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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RFP Dynamin2 K44A

Plasmid

#128153

Purpose

Expression of Dynamin 2 K44A

Depositor

Jennifer Lippincott-Schwartz

Article

Chua et al Proc Natl Acad Sci U S A. 2009 Dec 8;106

Insert

Dynamin 2 (Dnm2 Rat)

Expression

Mammalian

Mutation

K44A

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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RFP Dynamin2 Wild Type

Plasmid

#128152

Purpose

Expression of Dynamin 2

Depositor

Jennifer Lippincott-Schwartz

Article

Chua et al Proc Natl Acad Sci U S A. 2009 Dec 8;106

Insert

Dynamin 2 (Dnm2 Rat)

Expression

Mammalian

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-hTERT

Plasmid

#1771

Purpose

Retroviral expression of hTERT. Used to create immortalized cell lines.

Depositor

Bob Weinberg

Article

Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

Insert

hTERT (TERT Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS-HA

Plasmid

#130910

Purpose

Expresses human cGAS-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-mNeongreen

Plasmid

#182389

Purpose

Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHIV-iRFP720-E2A-Luc

Plasmid

#104587

Purpose

Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

Depositor

Antonius Plagge

Article

Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

Insert

iRFP720-E2A-Luciferase

Use

Lentiviral and Luciferase

Tags

bi-cistronic

Expression

Mammalian

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EGFP-IRES-puro

Plasmid

#128652

Purpose

mammalian expression of EGFP with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

EGFP

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgNeg1

Plasmid

#166998

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 1

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgNeg2

Plasmid

#166999

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 2

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-GFP

Plasmid

#78546

Purpose

Fusion protein Cas9+GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

Fusion protein with Cas9

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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beta-arrestin1 mYFP

Plasmid

#36916

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

beta-arrestin1 (Arrb1 Rat)

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAL119-TK

Plasmid

#21911

Depositor

Maria Castro

Article

Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

Insert

HSV Type 1 thymidine kinase

Use

Adenoviral

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-mTurquoise2

Plasmid

#108235

Purpose

Broad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

Pc promoter - mTurquoise2

Expression

Bacterial

Mutation

codon optimized for experession in P. fluorescens

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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