We narrowed to 9,847 results for: try
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Plasmid#138180PurposeExpresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only
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pBP-lacZ
Plasmid#72948PurposeEntry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloningDepositorInsertLevel 0 - Bioparts
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
plk1 T210D
Plasmid#132964PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
plk1 T210A
Plasmid#132965PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2R3z-AtU3b-tRNA-ccdB-gRNA
Plasmid#118390PurposeEntry clone expressing gRNA. Target site can be introduced between tRNA and gRNA backbone. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertAtU3b promoter
UseCRISPRAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
p221z-CAS9p-TagRFP-t35s
Plasmid#118386PurposeEntry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertCAS9p-TagRFP
UseCRISPRAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133B2.0
Plasmid#99892PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU3Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2R3z-BASI-ccdB-BSAI
Plasmid#118389PurposeEntry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertccdB
UseUnspecifiedMutationSee Depositor Comments SectionAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCx55.5-TrpR
Plasmid#86373Purposefor zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherryDepositorInsertTrpR
UseZebrafishPromotercx55.5Available SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-mVenus-CAAX
Plasmid#75153PurposeMembrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.DepositorInsertmVenus-CAAX
UseMiddle entry vector for multisite gateway three f…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
iTol2Amp-γ-crystallin:venusGFP
Plasmid#108462PurposeThis plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.DepositorInsertiTol2Amp crystGFP
Available SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ GFP-2xESP3I-IRES-mCherry
Plasmid#230996PurposeProtein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.DepositorTypeEmpty backboneTagsAcGFP1ExpressionMammalianPromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
SaCas9_sgRNA_expression_in_pBluescript
Plasmid#122090PurposeU6 driven SaCas9 sgRNA expression vector for cloning own guidesDepositorAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-Gal80ts
Plasmid#51619Purposevertebrate expression of full-length temperature-sensitive Gal80 with stopDepositorInsertGal80ts (GAL80 Budding Yeast)
UseVertebrate expressionMutationtemperature sensitive (G203V, G323R)*Available SinceMarch 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMuLE (R4-R3) PRB
Plasmid#206164PurposepMuLE R4-R3 entry plasmid containing human full length PRB gene, for 3-component Gateway assemblyDepositorAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-NICD1
Plasmid#46048DepositorInsertNotch1 (C-term) (Notch1 Mouse)
UseEntry vectorTagsMyc tagMutationCytoplasmic domain initiating at amino acid 1753Available SinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLG327
Plasmid#128797PurposeXylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.DepositorAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2
Plasmid#12291DepositorInsertTLR2 (Tlr2 Mouse)
TagsFLAG (FLAG-M2)ExpressionMammalianMutationM82I and H202Y; signal peptide (aa1-23) replaced …Available SinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pME-NR2F2
Plasmid#138359PurposeGateway compatible middle entry clone contains human full length NFR2F2 gene with stop codonDepositorInsertHuman NR2F2
ExpressionMammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only