We narrowed to 17,340 results for: emb

1. pEF-mRuby3

   Plasmid

   #179730

   Purpose

   Mammalian expression of mRuby3 from the EF1 promoter

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   mRuby3
   

   Expression

   Mammalian

   Available Since

   March 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMaCTag-07

   Plasmid

   #124786

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mNeonGreen
   

   Use

   Pcr template

   Tags

   mNeonGreen

   Promoter

   None

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTn7-SCOUT21.RY

   Plasmid

   #201006

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm-mCherry-sYFP2, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   mcherry
   sYFP2
   

   Expression

   Bacterial

   Promoter

   J23104

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTn7-SCOUT21.YB

   Plasmid

   #201008

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm-sYFP2-TagBFP, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   sYFP2
   TagBFP
   

   Expression

   Bacterial

   Promoter

   J23104

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET-His-APEX2

   Plasmid

   #201471

   Purpose

   Recombinant soluble APEX2 for SLAPSHOT labeling

   Depositor

   Yuh-Nung Jan

   Article

   Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

   Insert

   APEX2
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFBNb-cALFA

   Plasmid

   #159990

   Purpose

   For the protein expression with ALFA tag at C-terminus in insect cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Insect

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-cdc2 (716)

   Plasmid

   #61840

   Purpose

   mammalian expression of cdc2

   Depositor

   Jonathon Pines

   Article

   Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

   Insert

   cdc2 (CDK1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pDL1

   Plasmid

   #182263

   Purpose

   Gibson cloning vector for synthesis of single and double stranded RNA

   Depositor

   Daniel Lobo

   Article

   Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

   Type

   Empty backbone

   Use

   Gibson cloning vector for synthesis of single and…

   Promoter

   T3 and SP6 for sense and antisense riboprobe synt…

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Zeo-V5

   Plasmid

   #207708

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-V5 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pVENUS-N1-POLR2G-GFP

    Plasmid

    #86872

    Purpose

    To express POLR2G (RPB7) tagged with VENUS at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    POLR2G (POLR2G Human)
    

    Tags

    VENUS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PlayBack-Ef1a-Bamh1

    Plasmid

    #203308

    Purpose

    For expression of eukaryotic genes of interest with Ef1a promoter with Bamh1 as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOGG026

    Plasmid

    #113992

    Purpose

    Level 1 golden gate cloning vector for low copy number stable environmental gene expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TagBFP2-PIP4K2C

    Plasmid

    #202730

    Purpose

    expresses fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    mTagBFP2:PIP4K2C (PIP4K2C Human)
    

    Expression

    Mammalian

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTK81_GFP-4.1R

    Plasmid

    #46352

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1R (EPB41 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    pBABE

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRc/CMV HA TrkA

    Plasmid

    #39977

    Depositor

    Yves-Alain Barde

    Article

    Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

    Insert

    TrkA (Ntrk1 Rat)
    

    Tags

    BM40 signal sequence and HA

    Expression

    Mammalian

    Mutation

    endogenous signal sequence was replaced with BM40…

    Promoter

    CMV

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. C232-E05: attB2r-meGFP-attB3

    Plasmid

    #162902

    Purpose

    Gateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric enhanced Green fluorescent protein
    

    Use

    Synthetic Biology

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. C125-E01: stuffer

    Plasmid

    #162900

    Purpose

    Standard Gateway entry clone containing a non-transcribed/translated region for use in generating negative control vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    stuffer fragment for vector controls
    

    Use

    Synthetic Biology

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOGG024

    Plasmid

    #113991

    Purpose

    Level 1 golden gate cloning vector for medium copy number free-living expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. TOPO mTagBFP2

    Plasmid

    #68413

    Purpose

    TOPO construct expressing mTagBFP2 for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    mTagBFP2
    

    Use

    Gmap

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-C-moxGFP-Blast

    Plasmid

    #207710

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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