We narrowed to 11,785 results for: emb

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AA183

Plasmid

#216025

Purpose

Fragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiency

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

Cloning Grade DNA

Insert

nCas9 (H840A)_v2.1 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA028

Plasmid

#216008

Purpose

Fragmid fragment: (Cas protein) nickase Cas for base editing

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

Cloning Grade DNA

Insert

nCas9 (D10A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA060

Plasmid

#216017

Purpose

Fragmid fragment: (Cas protein) nickase Cas for prime editing

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

Cloning Grade DNA

Insert

nCas9 (H840A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-mRuby3

Plasmid

#214923

Purpose

expression vector control - contitutive expression of mRuby33 alone

Depositor

Ophir Shalem

Publication

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

mRuby3

Use

Lentiviral

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLP-sfgfp

Plasmid

#209994

Purpose

Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Publication

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

sfGFP

Expression

Bacterial

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pL0_CD-greenlantern

Plasmid

#202141

Purpose

Level 0 plasmid containing a green lantern fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Green lantern gene

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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EGFP-Scarlet-I

Plasmid

#193554

Purpose

Expresses mEGFP-mScarlet-I in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mScarlet-I

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-ShadowY

Plasmid

#193557

Purpose

Expresses mTurquoise2-ShadowY in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mTurquoise2-ShadowY

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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miniSOG-GFP

Plasmid

#185580

Purpose

for CALI

Depositor

Mamiko Yajima

Publication

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

miniSOG

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pYI001

Plasmid

#170833

Purpose

(GUSPlus::tOCS in pFP100)*

Depositor

Christian Fankhauser

Publication

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

GUSPlus::tOCS

Expression

Plant

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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L2-AA012

Plasmid

#185751

Purpose

Vector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

Depositor

Peter Szoevenyi

Publication

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Inserts

HygR2
eGFP

Tags

membrane localization tag Lti6b

Expression

Plant

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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MBP-RGG-LgBit-RGG

Plasmid

#176643

Purpose

LgBit fused to LAF-1 RGG domain with N-terminal MBP tag

Depositor

Daniel Hammer

Publication

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

MBP-RGG-LgBit-RGG

Expression

Bacterial

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E17: mPlum

Plasmid

#162897

Purpose

Standard Gateway entry clone encoding mPlum fluorescent protein

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Plum fluorescent protein

Use

Synthetic Biology

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pN23-LIC-His-FLAG

Plasmid

#165478

Purpose

pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His

Depositor

Renato Bruni

Publication

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-TEV

Expression

Bacterial

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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GGDestX1-Amp

Plasmid

#157652

Purpose

Golden Gate assembly and cell-free (bacterial) expression of fusion-proteins

Depositor

Karmella Haynes

Publication

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Cell-free expression (e. coli sigma-70)

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-686 (attR4-attR2)

Plasmid

#161889

Purpose

Multisite Gateway (attR4-attR2) vector for lentivirus production

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEG BacMam N term myc eGFP 3C

Plasmid

#160682

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Publication

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

myc eGFP 3C

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSM04

Plasmid

#139046

Purpose

MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mkate2 (no STOP codon)

Expression

Worm

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK130

Plasmid

#110584

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

T7 promoter with lac operator and RBS

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Pado Red pcDNA3.1

Plasmid

#79665

Purpose

Pado with mKate2

Depositor

Bradley Baker

Publication

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

Pado mKate2

Expression

Mammalian

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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