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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,416 results for: sequence

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Showing: 5961 - 5980 of 13416 results

  4. AICSDP-36: AAVS1-mEGFP (PGK)

    Plasmid
    #114404
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114404-plasmid-map-sequence-id-220815

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  5. pJYDC1

    Plasmid
    #162458
    Purpose
    Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the Nterminus
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA tag, HDEL sequence, eUnaG2 fluorescent protein…
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162458-plasmid-map-sequence-id-321474

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  8. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201152
    Purpose
    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    dCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: TACGTTCTCTATCACTGAT
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201152-plasmid-map-sequence-id-401799

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  12. AICSDP-52: HIST1H2BJ-mEGFP

    Plasmid
    #109121
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJ
    Depositor
    Allen Institute for Cell Science
    Insert
    HIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    109121-plasmid-map-sequence-id-209767

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  14. AICSDP-26:ST6GAL1-mEGFP

    Plasmid
    #101786
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1
    Depositor
    Allen Institute for Cell Science
    Insert
    ST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101786-plasmid-map-sequence-id-197675

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  16. pGFP Cas

    Plasmid
    #50729
    Purpose
    mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    Cas (Bcar1 Rat)
    Tags
    FLAG and GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50729-plasmid-map-sequence-id-340937

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  18. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Expression
    Bacterial and Mammalian
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    113631-plasmid-map-sequence-id-222216

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Showing: 5961 - 5980 of 13416 results