We narrowed to 853 results for: Tg

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pCRIS-PITChv2-dTAG-Puro (BRD4)

Plasmid

#91796

Purpose

PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

FKBP_F36V-2xHA-P2A-Puro

Use

CRISPR

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promotorless

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCRIS-PITChv2-BSD-dTAG (BRD4)

Plasmid

#91792

Purpose

PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

BSD-P2A-2xHA-FKBP_F36V

Use

CRISPR

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promoterless

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEM-HE-h_bPAC_cmyc

Plasmid

#28134

Purpose

optogenetic tool for light modulation of cellular cAMP

Depositor

Peter Hegemann

Article

Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

Insert

humanized photoactivated adenylyl cyclase

Use

In vitro transcription

Tags

Myc

Available Since

June 23, 2011

Availability

Academic Institutions and Nonprofits only

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pCRIS-PITChv2-dTAG-BSD (BRD4)

Plasmid

#91795

Purpose

PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

FKBP_F36V-2xHA-P2A-BSD

Use

CRISPR

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promotorless

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

Plasmid

#101060

Purpose

AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 neo

Plasmid

#98292

Purpose

Variant of lentiCRISPRv2 that confers G418 resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pHis-hTG2

Plasmid

#100719

Purpose

Expression of human transglutaminase 2 in E.coli

Depositor

Byung Il Lee

Article

Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

Insert

TGM2 (TGM2 Human)

Tags

His

Expression

Bacterial

Mutation

silent mutation Arg580 (AGG--CGT) to enhance expr…

Promoter

T7

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 hygro

Plasmid

#98291

Purpose

Variant of lentiCRISPRv2 that confers hygromycin resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 blast

Plasmid

#98293

Purpose

Variant of lentiCRISPRv2 that confers blasticidin S resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pX330A-nBRD4/PITCh

Plasmid

#91794

Purpose

PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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