We narrowed to 1,449 results for: keith joung

1. miniABEmax (pRZ900)

   Plasmid

   #131311

   Purpose

   CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CGBE1 (pRZ3885)

   Plasmid

   #140252

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAC-BA-lacZ

   Plasmid

   #13423

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Insert

   BA
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. MMLV-RT(dRH) for Split-PE (pEH50)

   Plasmid

   #190106

   Purpose

   Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   MMLV-RT(delta RNAseH)
   

   Use

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   mutations from RT in PE2 and truncation of RNAse …

   Promoter

   CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK2660

   Plasmid

   #70709

   Purpose

   Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. CGBE1-NG (pBM1246)

   Plasmid

   #140256

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)_NG-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TAL2065

   Plasmid

   #39435

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   eGFP-TALEN-39-Right
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

8. TAL2064

   Plasmid

   #39434

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   eGFP-TALEN-39-Left
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

9. MSP1830

   Plasmid

   #70708

   Purpose

   Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Insert

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   CAG

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. miniCGBE1-SpRY (pBM1571)

    Plasmid

    #170105

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…

    Promoter

    CMV

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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