We narrowed to 769 results for: ELP

1. pHelper-T7IS1

   Plasmid

   #140631

   Purpose

   Expresses ShCAST under the T7 promoter and an empty sgRNA

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. tdTomato-ELP-1-25

   Plasmid

   #58091

   Purpose

   Localization: Golgi/ER, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Insert

   ELP (KDELR2 Human)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pEBG-PELP1

   Plasmid

   #65209

   Purpose

   Full length mammalian expression vector for hPELP1

   Depositor

   Ratna Vadlamudi

   Article

   Nair et al Cancer Res. 2010 Sep 15;70(18):7166-75.

   Insert

   PELP1 (PELP1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. ECFP-ELP1-25

   Plasmid

   #55341

   Purpose

   Localization: Golgi/ER, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Insert

   ELP1 (KDELR2 Human)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. mTFP1-ELP-1-25

   Plasmid

   #55478

   Purpose

   Localization: Golgi/ER, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   ELP (KDELR2 Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pET22b-EK-cleavableELP

   Plasmid

   #219404

   Purpose

   Enterokinase-responsive elastin-like polypeptide (ELP)

   Depositor

   Danielle Mai

   Article

   Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

   Insert

   EK-cleavableELP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. POLN delP_pDEST17

   Plasmid

   #64886

   Purpose

   bacterial expression of human POLN with a short truncation of the C-terminal proline-rich tail

   Depositor

   Richard Wood

   Article

   Takata et al J Biol Chem. 2006 Aug 18;281(33):23445-5

   Insert

   POLN (POLN Human)
   

   Use

   Tags

   Flag and His

   Expression

   Bacterial

   Mutation

   with a short truncation of the C-terminal proline…

   Promoter

   T7

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. rAAV2-retro helper

   Plasmid

   #81070

   Purpose

   retrograde AAV helper vector, carrying rep and modified capsid gene

   Depositor

   David Schaffer, Alla Karpova

   Article

   Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

   Inserts

   rep
   rAAV2-retro cap
   

   Use

   AAV and Unspecified; Helper vector

   Tags

   Expression

   Mutation

   Pro2Ala, possibly Asp613Gly and changes made to A…

   Promoter

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. mini-pHelper-1.0

   Plasmid

   #230933

   Purpose

   The mini verison (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

   Depositor

   Qizhao Wang

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET/ELP-I-CAT

    Plasmid

    #71461

    Purpose

    ELP-intein purification system

    Depositor

    David Wood

    Article

    Banki et al Nat Methods. 2005 Sep;2(9):659-61.

    Type

    Empty backbone

    Use

    Tags

    ELP ((VPGXG)110) and intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-24a-ELP[V-30]

    Plasmid

    #67014

    Purpose

    Expresses ELP[V-30] in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    ELP[V-30]
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAL-c5X-ELP[AV-60]

    Plasmid

    #67012

    Purpose

    Expresses malE-ELP[AV-60] in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-ELP[AV-60]
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. SELP-bio-His

    Plasmid

    #51639

    Purpose

    Expresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SELP (SELP Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tn7 helper plasmid

    Plasmid

    #141161

    Purpose

    Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

    Depositor

    Shimon Bershtein

    Article

    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

    Insert

    Tn7 transposon machinery
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose-inducible pBAD

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET22b-TEVP-cleavableELP

    Plasmid

    #219402

    Purpose

    Tobacco etch virus protease-responsive elastin-like polypeptide (ELP)

    Depositor

    Danielle Mai

    Article

    Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

    Insert

    TEVP-cleavableELP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ELP-TEMP/pcDNA3.1(-)

    Plasmid

    #178434

    Purpose

    Genetically encoded ratiometric fluorescent thermometer

    Depositor

    Takeharu Nagai

    Article

    Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

    Insert

    ELP-TEMP
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-A96 ELP

    Plasmid

    #67856

    Purpose

    GFP Fluorescent Elastin-like Polypeptide(VPGAG) with 96 ELP repeats containing Alanine guest residue

    Depositor

    Andrew MacKay

    Insert

    GFP fused to ELP (VPGAG) repeated 96 times
    

    Use

    Tags

    GFP fused to N-terminal of ELP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMAL-c5X-ELP[V-60]

    Plasmid

    #67013

    Purpose

    Expresses malE-ELP[V-60] in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-ELP[V-60]
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHelper-CBE-sgRNA

    Plasmid

    #221137

    Purpose

    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    sgRNA-scaffold with Cas9 handle
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI)

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-V96 ELP

    Plasmid

    #67853

    Purpose

    GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

    Depositor

    Andrew MacKay

    Article

    Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

    Insert

    GFP fused to ELP (VPGVG) repeated 96 times
    

    Use

    Tags

    GFP fused to N-terminal of ELP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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