Addgene

We narrowed to 2,823 results for: GEM

Showing: 41 - 60 of 2823 results

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pGEM-HE-h_bPAC_cmyc

Plasmid

#28134

Purpose

optogenetic tool for light modulation of cellular cAMP

Depositor

Peter Hegemann

Article

Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

Insert

humanized photoactivated adenylyl cyclase

Use

In vitro transcription

Tags

Myc

Expression

Mutation

Promoter

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only

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pGEM-3z/602

Plasmid

#26657

Depositor

Jonathan Widom

Article

Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only
pGEM2 hTSP-1

Plasmid

#12993

Depositor

Jack Lawler

Insert

TSP-1 (THBS1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

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pGEM5Z(+)-EGFP

Plasmid

#65206

Purpose

EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.

Depositor

LuZhe Sun

Article

Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

Insert

EGFP

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Restriction enzyme recognition sites added either…

Promoter

CMV

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only
pCS2 Geminin

Plasmid

#12174

Depositor

Marc Kirschner

Article

McGarry et al Cell. 1998 Jun 12. 93(6):1043-53.

Insert

Geminin (gmnn Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2006

Availability

Academic Institutions and Nonprofits only
pGEMTEZ-TeTxLC

Plasmid

#32640

Depositor

C. Ron Yu, Richard Axel, Joseph Gogos

Article

Yu et al Neuron. 2004 May 27;42(4):553-66.

Insert

Tetanus toxin light chain

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pGEMTEZ-Kir2.1

Plasmid

#32641

Depositor

C. Ron Yu, Richard Axel, Joseph Gogos

Article

Yu et al Neuron. 2004 May 27;42(4):553-66.

Insert

Kir2.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pCas9-HE-Geminin

Plasmid

#109402

Purpose

Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

HE-Geminin

Use

Tags

Expression

Mutation

Promoter

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGEM-Sox2

Plasmid

#16353

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pGEM-Oct4

Plasmid

#16352

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pCas9-Geminin

Plasmid

#109401

Purpose

Expresses Cas9 fusion to Geminin degron domain

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

Geminin (GMNN Human)

Use

Tags

Expression

Mutation

Geminin degron fragment

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGEM4Z-mMcl-1

Plasmid

#32984

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pGEM p21

Plasmid

#8443

Depositor

Frederic Mushinski

Article

Huppi et al Oncogene 1994 Oct;9(10):3017-20.

Insert

Mouse p21 (Cdkn1a Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pQdCas9-sgempty

Plasmid

#159523

Purpose

Expression of dCas9 driven by lux cassette from Vibrio fischeri

Depositor

Radhakrishnan Mahadevan

Article

Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

Insert

quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

quorum sensing promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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RhGC(BE)_pGEM

Plasmid

#85469

Purpose

The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

Depositor

Peter Hegemann

Article

Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

Insert

rhodopsin-guanylyl cyclase

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGEM-LIN28

Plasmid

#16350

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens lin-28 homolog (C. elegans) (LIN28A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pGEMT-3P2A

Plasmid

#111772

Purpose

Quadcistronic construct: Co-express four genes by 3 P2A peptides

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Type

Empty backbone

Use

Cloning intermediate

Tags

Expression

Mutation

Promoter

T7

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer:cAPX

Plasmid

#84736

Purpose

Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

APX1 (APX1 Mustard Weed)
HyPer2 targeted to nucleus

Use

Tags

NLS-SV40

Expression

Plant

Mutation

Promoter

pFMV/p35S

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGem-sHyper:sAPX

Plasmid

#84738

Purpose

Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

sAPX (SAPX Mustard Weed)
sHyper (HyPer2 targeted to nucleus)

Use

Tags

nuclear localisation signals from the coding sequ…

Expression

Plant

Mutation

Promoter

pFMV/p35S

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer:sAPX

Plasmid

#84735

Purpose

Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

sAPX (SAPX Mustard Weed)
nHyper (HyPer2 targeted to nucleus)

Use

Tags

nuclear localisation signals from the coding sequ…

Expression

Plant

Mutation

Promoter

FMV/35S

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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