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Plasmid#106263PurposePolycistronic expression of luc, ekivR using P2ADepositorInsertluc-P2A-ekivR
UseLuciferase and Synthetic BiologyTagsExpressionMutationPromoterTet-OnAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS2163
Plasmid#169632PurposeTier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)DepositorInsertOTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3
UseTagsExpressionMammalianMutationPromotertetO7-PhCMVmin / PPGKAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSA-38
Plasmid#17328DepositorInsertstreptavidin
UseTagsExpressionBacterialMutationTruncated to include only amino acids 16-133 of m…PromoterAvailable sinceFeb. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTSN7A
Plasmid#131319PurposeExpressing synthetic TetR-GFP protein in N. crassa (from the his-3 locus)DepositorInsertTetR-GFP
UseUnspecifiedTagsEGFPExpressionMutationPromoterTrpC (from pCSN44)Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS32.372
Plasmid#106262PurposePolycistronic expression of luc, ekivR, esyn1 using P2ADepositorInsertluc-P2A-ekivR-P2A-esyn1
UseLuciferase and Synthetic BiologyTagsExpressionMutationPromoterTet-OnAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pTSN7B
Plasmid#131320PurposeExpressing synthetic TetR-GFP protein in N. crassa (from the csr-1 locus)DepositorInsertTetR-GFP
UseUnspecifiedTagsEGFPExpressionMutationPromoterTrpC (from pCSN44)Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.6t
Plasmid#210258PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmScarlet
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.7t
Plasmid#210259PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertse2Crimson
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.3t
Plasmid#210255PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsDsRed
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSK405
Plasmid#190817PurposeFor C-terminus 3xGFP tagging of a gene of interest using URA3 selection marker in S. cerevisiaeDepositorInsertURA3 (URA3 Budding Yeast)
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSK573
Plasmid#190881PurposeFor C-terminal -Halo Tagging of a gene of interest with TRP1 selection marker in yeast S. cerevisiaeDepositorInsertHaloTag
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2326
Plasmid#169628PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2321
Plasmid#169626PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1037
Plasmid#169616PurposeTier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-pA).DepositorInsertPPGK-driven expression of secreted Nluc
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2329
Plasmid#169629PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1043
Plasmid#169621PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .DepositorInsertPCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1041
Plasmid#169619PurposeTier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2322
Plasmid#169627PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and Tactb-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2320
Plasmid#169625PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only