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Showing: 41 - 60 of 198 results

  1. pSecUAG_UTu1D

    Plasmid
    #233867
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistant
    Depositor
    Natalie Krahn, Dieter Söll
    Inserts
    allo-tRNA UTu1D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG) and …
    Promoter
    EM7, araBAD, and native As SelD
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233867-plasmid-map-sequence-id-471093

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  2. pET15bHis6-pG-Tnp

    Plasmid
    #124884
    Purpose
    To transform BL21-Gold(DE3) and induced by IPTG for His-proteinG-Tnp (hyperactive mutant) fusion protein.
    Depositor
    Keji Zhao
    Insert
    protein G's C1D1C2D2C3 domains contained in the protein G gBLOCK.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    124884-plasmid-map-sequence-id-243752

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  3. pET-Impact-T4gp32(L301G)

    Plasmid
    #228209
    Purpose
    Expression in BL21(DE3) of single strand DNA binding protein L301G
    Depositor
    Stephen Benkovic
    Insert
    61
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated leucine 301 to glycine (L301G), improves …
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228209-plasmid-map-sequence-id-453946

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  10. pETMCSI-T4P_Y320A

    Plasmid
    #122522
    Purpose
    Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis
    Depositor
    Gottfried Otting
    Article
    Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do
    Insert
    gp43 - DNA polymerase (43 Escherichia virus T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tyrosine 320 changed to alanine
    Promoter
    T7 promoter
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122522-plasmid-map-sequence-id-238200

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  12. RepX_pET28a(+)

    Plasmid
    #172199
    Purpose
    Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.
    Depositor
    Taekjip Ha
    Article
    Arslan et al Science. 2015 Apr 17;348(6232):344-7. do
    Insert
    RepX superhelicase
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    C18L, C43S, C167V, S400C, C612A
    Promoter
    Available since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    172199-plasmid-map-sequence-id-356977

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  13. pET28a_LolT_C41S

    Plasmid
    #211789
    Purpose
    Expression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41S
    Depositor
    Yang Hai
    Article
    Gao et al Chembiochem. 2023 Oct 1:e202300561. doi:
    Insert
    lolt mutant
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    211789-plasmid-map-sequence-id-420926

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  15. pOGG030

    Plasmid
    #113993
    Purpose
    Level 0 module. IPTG-inducible T7RNAP promoter for BL21 protein purification
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    IPTG-inducible T7RNAP promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113993-plasmid-map-sequence-id-223386

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Showing: 41 - 60 of 198 results