We narrowed to 91,060 results for: sap

1. pcDNA3-sapphireCKAR(T/A)

   Plasmid

   #118469

   Purpose

   Negative-control mutant for sapphireCKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireCKAR(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPICZalphaB-SapL3

   Plasmid

   #78171

   Purpose

   Expression of Saporin L3 in pichia

   Depositor

   Vern Schramm

   Article

   Yuan et al Biochemistry. 2015 Jul 28;54(29):4565-74

   Insert

   Saporin L3
   

   Use

   Tags

   alpha factor

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-postASAP

   Plasmid

   #178792

   Purpose

   Expresses voltage sensor targeted to dendrites and spines

   Depositor

   Rafael Yuste

   Article

   Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

   Insert

   FingR.PSD95 and ASAP2f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutations in amino acids L146G, S147T N149R, S150…

   Promoter

   CAG

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOpen-SapIR

   Plasmid

   #165537

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.

   Depositor

   FreeGenes Project, Drew Endy, Jennifer Molloy

   Insert

   SapI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. Sirius-mT-Sapphire_pcDNA3

   Plasmid

   #89584

   Purpose

   Genetically encoded ratiometric fluorescent thermometer

   Depositor

   Takeharu Nagai

   Article

   Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

   Insert

   Sirius-mT-Sapphire
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mT-Sapphire-pBAD

   Plasmid

   #54571

   Purpose

   Localization: Protein Expression Vector , Excitation: 399, Emission: 511

   Depositor

   Michael Davidson, Robert Campbell

   Article

   Ai et al Nat Methods. 2008 May . 5(5):401-3.

   Type

   Empty backbone

   Use

   Tags

   6xHis and mT-Sapphire

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. fSAP24-GST

   Plasmid

   #11202

   Depositor

   Robin Reed

   Article

   Masuda et al Genes Dev. 2005 Jul 1. 19(13):1512-7.

   Insert

   SAP24 (THOC7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pCAG-HASAP

   Plasmid

   #138325

   Purpose

   chemigenetic voltage indicator

   Depositor

   Eric Schreiter

   Article

   Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

   Insert

   HASAP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLARRYv2-T-Sapphire

   Plasmid

   #233209

   Purpose

   Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with T-Sapphire marker

   Depositor

   Alejo Rodriguez-Fraticelli

   Article

   Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCVpuro-Sapphire

    Plasmid

    #96950

    Purpose

    Retroviral plasmid containing Sapphire for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    Sapphire fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV3Tag8li-hSAPS1-HA

    Plasmid

    #165466

    Purpose

    Human SAPS1 mammalian expression vector with C-terminal HA tag.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    Protein phosphatase 6 regulatory subunit 1 (PPP6R1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEB1-T-Sapphire

    Plasmid

    #103977

    Purpose

    Plasmid encoding T-Sapphire

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    T-Sapphire
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (Q69M, C70V, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSaPIPLC His

    Plasmid

    #173810

    Purpose

    Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Anne Gershenson, Mary F Roberts

    Article

    Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

    Insert

    Staphylococcus aureus plc
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-HASAP1-ST

    Plasmid

    #138965

    Purpose

    chemigenetic voltage indicator, soma targeted

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Insert

    HASAP1-ST
    

    Use

    Tags

    somatic localization tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEB1-Sapphire

    Plasmid

    #103976

    Purpose

    Plasmid encoding Sapphire

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    Sapphire
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S72A, Y145F,…

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+)-sapphireAKAR

    Plasmid

    #118464

    Purpose

    cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGem-sHyper:sAPX

    Plasmid

    #84738

    Purpose

    Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Inserts

    sAPX (SAPX Mustard Weed)
    sHyper (HyPer2 targeted to nucleus)
    

    Use

    Tags

    nuclear localisation signals from the coding sequ…

    Expression

    Plant

    Mutation

    Promoter

    pFMV/p35S

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGem-nHyPer:sAPX

    Plasmid

    #84735

    Purpose

    Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Inserts

    sAPX (SAPX Mustard Weed)
    nHyper (HyPer2 targeted to nucleus)
    

    Use

    Tags

    nuclear localisation signals from the coding sequ…

    Expression

    Plant

    Mutation

    Promoter

    FMV/35S

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mT-Sapphire-CytERM-N-17

    Plasmid

    #56278

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 511

    Depositor

    Michael Davidson

    Insert

    CytERM
    

    Use

    Tags

    mT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pRiboI SapNde

    Plasmid

    #54490

    Purpose

    Theophylline-inducible expression in single-copy integrating vector

    Depositor

    Jessica Seeliger

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    hsp60

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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