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We narrowed to 11,183 results for: NSI

Showing: 1161 - 1200 of 11183 results

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ORF24-2xStrep pCDNA4

Plasmid

#129742

Purpose

Expresses KSHV ORF24 with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF24 (ORF24 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

2xStrep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-roGFP1-iE

Plasmid

#83312

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

Insert

roGFP1-iE

Use

Tags

6xHIS

Expression

Bacterial

Mutation

ro1(C48S/S147C/Q204C) plus X insertion, iE betwe…

Promoter

T5

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-Xl-VSP1 C301S

Plasmid

#51882

Purpose

expression of Xl-VSP1 inactive mutant in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 C301S (tpte2 Frog)

Use

Xenopus oocyte expression

Tags

Expression

Mammalian

Mutation

C301S, catalytically-inactive mutant of Xl-VSP1

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pMCh-1468

Plasmid

#82418

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis GW182 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvGW182

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-CS1M2b-3c-ON8-EGFP

Plasmid

#228452

Purpose

LS4-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS1M2b-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-CS2-3c-ON8-EGFP

Plasmid

#228453

Purpose

LS12-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS2-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-evoBxb1

Plasmid

#232747

Purpose

IVT template for making modRNA encoding evoBxb1

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Expression

Mutation

V74A

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-Bxb1-I87L

Plasmid

#232749

Purpose

IVT template for making modRNA encoding mutant Bxb1 modRNA (I87L)

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Expression

Mutation

I87L

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-Bxb1

Plasmid

#232746

Purpose

IVT template for making modRNA encoding Bxb1

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Expression

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-PuroR

Plasmid

#232753

Purpose

IVT template for making modRNA encoding the Puromycin resistance gene

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

PuroR

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-SP5

Plasmid

#232653

Purpose

transient expresses SP5 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

SP5 (SP5 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-SP2

Plasmid

#232652

Purpose

transient expresses SP2 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

SP2 (SP2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S2-mRuby2-Vt

Plasmid

#233227

Purpose

Vinculin FRET tension sensor with coiled-coil S2 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S2-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-M2-mRuby2-Vt

Plasmid

#233231

Purpose

Vinculin FRET tension sensor with coiled-coil M2 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-M2-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S1-mRuby2-Vt

Plasmid

#233230

Purpose

Vinculin FRET tension sensor with coiled-coil S1 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S1-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S0-mRuby2-Vt

Plasmid

#233226

Purpose

Vinculin FRET tension sensor with coiled-coil S0 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S0-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S4-mRuby2-Vt

Plasmid

#233228

Purpose

Vinculin FRET tension sensor with coiled-coil S4 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S4-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-S7-mRuby2-Vt

Plasmid

#233229

Purpose

Vinculin FRET tension sensor with coiled-coil S7 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S7-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-Mad-mRuby2-Vt

Plasmid

#233232

Purpose

Vinculin FRET tension sensor with coiled-coil Mad domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-Mad-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_TRE-mScarlet

Plasmid

#229796

Purpose

Bxb1-GT donor plasmid for doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mScarlet

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_CAG-mStayGold

Plasmid

#229802

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_TRE-jRCaMP1b

Plasmid

#229798

Purpose

Bxb1-GA donor plasmid for doxycycline-inducible expression of jRCaMP1b calcium reporter.

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

jRCaMP1b

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA RBS30 mscarlet

Plasmid

#226370

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

Depositor

Urartu Seker

Article

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

mScarlet

Use

Tags

6x Histag

Expression

Bacterial

Mutation

Promoter

copA promoter (pCopA)

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA sfgfp

Plasmid

#226374

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with native RBS (ribosome binding site).

Depositor

Urartu Seker

Article

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

sfGFP

Use

Tags

6x HisTag

Expression

Bacterial

Mutation

Promoter

pCopA

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac SOX R139A

Plasmid

#192058

Purpose

Expression vector for SOX (ORF37) R139A

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37)

Use

Tags

Expression

Insect

Mutation

R139A

Promoter

Polyhedrin

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACE WT SOX pACEmam1

Plasmid

#192049

Purpose

Expresses WT SOX (pACEmam1)

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

WT SOX

Use

Unspecified

Tags

Expression

Mutation

Promoter

CMV

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac SOX Q129H

Plasmid

#192057

Purpose

Expression vector for SOX (ORF37) Q129H

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37) Q129H

Use

Tags

Expression

Insect

Mutation

Q129H

Promoter

Polyhedrin

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDSK519-cspB

Plasmid

#207162

Purpose

Expresses codon-optimized cspB from Clavibacter michiganensis in a broad host range vector

Depositor

Gitta Coaker

Article

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspB

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

nptII

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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TtA_pET29b

Plasmid

#215432

Purpose

Expression construct for TtA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

TtA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTcI39-PRM-wasabi

Plasmid

#195555

Purpose

Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mWasabi
TcI39

Use

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

A67T

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD063

Plasmid

#212916

Purpose

Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-ORF29

Plasmid

#200028

Purpose

Lentiviral vector for expression of KSHV ORF29

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF29

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG.INBox-mCherry-INBox-eDHFR

Plasmid

#176203

Purpose

Double INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

INBox-mCherry-INBox-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG.mCherry-INBox-eDHFR

Plasmid

#176202

Purpose

Single INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-INBox-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG.mCherry-INBox(AAA)-eDHFR

Plasmid

#176207

Purpose

INBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-INBoxAAA-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-zeo-2xStrep-ORF68

Plasmid

#162659

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-zeo-2xStrep-UL52

Plasmid

#162663

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged UL52 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL52

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 C415A

Plasmid

#162633

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C415A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C415 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 H452A

Plasmid

#162634

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 H452A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid H452 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 C79A

Plasmid

#162635

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C79A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C79 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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