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Plasmid#112657PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K S389,398D
UseTagsExpressionYeastMutationR59K, mutations (S389D, S398D) in Glo3 motif, pho…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 ΔGRM-ΔC
Plasmid#112659PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 ΔGRM ΔC
UseTagssfGFPExpressionYeastMutationΔ376-493 (deletion of Glo3 motif & C-terminal…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 A
Plasmid#112660PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 S389,398A
UseTagssfGFPExpressionYeastMutationmutations (S389A, S398A) in Glo3 motif, non-phosp…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 D
Plasmid#112661PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 S389,398D
UseTagssfGFPExpressionYeastMutationmutations (S389D, S398D) in Glo3 motif, phosphomi…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K L246D
Plasmid#112648PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K L246D
UseTagsExpressionYeastMutationR54K & L246D (in ALPS motif)PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K AxxA
Plasmid#112649PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K AxxA
UseTagsExpressionYeastMutationR54K & Alanine substitution of C-terminal δL …PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 Δ3xF AxxA
Plasmid#112650PurposeExpression S.cerevisiaeDepositorInsertGcs1 Δ3xF AxxA
UseTagsExpressionYeastMutationR54K, W304A, W307A, W310A, W349A, F353APromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP569
Plasmid#139499PurposeRepair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiaeDepositorInsertpSTL1 24xPP7sl STL1 100 - 600
UseTagsExpressionYeastMutationWTPromoterpSTL1Available sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFPJ1
Plasmid#83561PurposeExpress yEGFP-ERG9 ER signal peptide under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER signal peptide
UseTagsExpressionYeastMutationPromoterTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
dRT-pMDLg/pRRE
Plasmid#60488Purposegag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)DepositorInsertHIV-1 gag, HIV-1 pol with inactive reverse transcriptase
UseTagsExpressionMutationchanged Asp at position 249 + 250 to ValPromoterAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)
Plasmid#86424PurposePCR tagging module with yeast codon optimized mTurquoise2DepositorInsertmTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYSD300
Plasmid#212930PurposeEncodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.DepositorInsertAGA1 (AGA1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
14x PP7 with loxP-kanMX-loxP
Plasmid#189939PurposeIntegration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)DepositorInsert14x PP7 with loxP-kanMX-loxP
UseTagskanMXExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-3HA
Plasmid#41608DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1
Plasmid#41605DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
ZJOM11
Plasmid#133637PurposeIntegration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorInsertpCUP1-GFP-Tlg1 (TLG1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1
Plasmid#41607DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GST
Plasmid#41611DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP
Plasmid#41614DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJC30X-BS(X)
Plasmid#58958PurposeEncodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorInsertEnhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
UseTagsFirst 8 codons of COX3ExpressionMutationGFP modified as described: CORMACK, B. P., R. H. …Promotern/aAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYSD153
Plasmid#212929PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD141
Plasmid#212928PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1-3HA
Plasmid#41610DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
ZJOM12
Plasmid#133638PurposeIntegration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorInsertpCUP1-GFP-Tlg2 (TLG2 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
BtA3N
Plasmid#238981PurposeIn vitro transcription of bovine arrestin-3 short isoformDepositorInsertarrestin-3 short (ARR3 Bovine)
UseIn vitro transcriptionTagsExpressionMutationsilent, Cla I side introduced before stop codonPromoterSP6Available sinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pYSD101
Plasmid#212925PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD043
Plasmid#212913PurposeEncodes the S. cerevisiae 111 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSCW4 (SCW4 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD084
Plasmid#212921PurposeEncodes the HA-tagged S. cerevisiae Tip1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertTIP1 (TIP1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD080
Plasmid#212917PurposeEncodes the S. cerevisiae Aga1p yeast surface display anchor protein as a Type 3 part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA1 (AGA1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD001
Plasmid#212898PurposeEncodes S.cerevisiae AGA2 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA2 (AGA2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD008
Plasmid#212905PurposeEncodes S. cerevisiae PLB1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertPLB1 (PLB1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD006
Plasmid#212903PurposeEncodes S. cerevisiae MFalpha1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD083
Plasmid#212920PurposeEncodes the HA-tagged S. cerevisiae Sed1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSED1 (SED1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD082
Plasmid#212919PurposeEncodes the HA-tagged S. cerevisiae Cwp2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertCWP2 (CWP2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD003
Plasmid#212900PurposeEncodes S.cerevisiae ECM14 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertECM14 (ECM14 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD002
Plasmid#212899PurposeEncodes S.cerevisiae CRH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertCRH1 (CRH1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD007
Plasmid#212904PurposeEncodes S. cerevisiae OST1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertOST1 (OST1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD041
Plasmid#212911PurposeEncodes the S. cerevisiae YAR066W Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertYAR066W (YAR066W Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD023
Plasmid#212909PurposeEncodes a hybrid S. cerevisiae OST1 pre- MFalpha1 pro- signal peptide (OST1MFapp) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD004
Plasmid#212901PurposeEncodes S.cerevisiae SUC2 (Invertase) pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD081
Plasmid#212918PurposeEncodes the HA-tagged S. cerevisiae Aga2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA2 (AGA2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD021
Plasmid#212907PurposeEncodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFapp8) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD024
Plasmid#212910PurposeEncodes the wildtype S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappWT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD042
Plasmid#212912PurposeEncodes the S. cerevisiae HSP150 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertHSP150 (HSP150 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD005
Plasmid#212902PurposeEncodes S. cerevisiae KSH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertKSH1 (KSH1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD061
Plasmid#212915PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD044
Plasmid#212914PurposeEncodes the S. cerevisiae 142 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSCW4 (SCW4 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD022
Plasmid#212908PurposeEncodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappOPT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD009
Plasmid#212906PurposeEncodes S. cerevisiae SRL1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSRL1 (SRL1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only