We narrowed to 28,654 results for: IGH@

1. pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB

   Plasmid

   #86194

   Purpose

   beta Globin NMD construct with and without PTC under Bi-directional TET on promoter

   Depositor

   Robert Singer

   Article

   Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

   Insert

   beta Globin (HBB, Hbb Mouse, Human)
   

   Use

   Tet on

   Tags

   Expression

   Mammalian

   Mutation

   39TER in beta Globin in MCS II

   Promoter

   Tet on

   Available since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV ArcLightCo (Q239)-T2A-nls-mCherry

   Plasmid

   #85806

   Purpose

   Genetically encoded voltage sensor ArcLight- codon optimized

   Depositor

   Vincent Pieribone

   Article

   Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

   Insert

   ArcLightCo-Q239
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   CMV

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-Tight-Puro FLAG-SMAD4 TetON

   Plasmid

   #107129

   Purpose

   Inducible expression of Flag-tagged SMAD4 in mammalian cells

   Depositor

   Joan Massague

   Article

   David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

   Insert

   SMAD4
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

   Plasmid

   #102890

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

   Plasmid

   #102892

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRetroX-Tight-puro-3xFLAG-RUNX3 R122C

   Plasmid

   #203439

   Purpose

   Mammalian expression of RUNX4

   Depositor

   Yoshiaki Ito

   Article

   Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

   Insert

   RUNX3 (RUNX3 Human)
   

   Use

   Tags

   3 x FLAG

   Expression

   Mammalian

   Mutation

   R122C

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

   Plasmid

   #102891

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSyn ArcLightCo (Q239)-T2A-nls-mCherry

   Plasmid

   #85844

   Purpose

   Genetically encoded voltage sensor ArcLight- codon optimized

   Depositor

   Vincent Pieribone

   Article

   Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

   Insert

   ArcLightCo-Q239
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   hSyn1

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tre-Tight ifgMosaic Donor vector (LH121)

   Plasmid

   #99630

   Purpose

   To clone the 3 ORFs for Tet system (rtTA + Dox) dependent generation of ifgMosaics

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   N-PhiM
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   (Please see depositor comments below)

   Promoter

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. XZ087 SFFV-mCherry-sensor(tight)-hIgG1-TM

    Plasmid

    #233428

    Purpose

    LIDAR reporter RNA with a membrane-bound human IgG as output

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    mCherry-sensor(tight)-hIgG1-TM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMVd1-NLS-GAL4-iLight-VP16

    Plasmid

    #219549

    Purpose

    NLS-Gal4(1-65)-iLight-VP16 construc expression in mammalian cells. pAAV vector.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

    Insert

    NLS-Gal4(1-65)-iLight-VP16
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVd1

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. vYM010_phiC31-nTRE-pEF1s(high)-H2b-Citrine

    Plasmid

    #197678

    Purpose

    "pEF1s(high)" reporter plasmid

    Depositor

    Michael Elowitz

    Article

    Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

    Insert

    pEF1s-H2B-Citrine
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TREtight-H2b-emiRFP670-P2A-TEVP

    Plasmid

    #174379

    Purpose

    AAV virus for expressing TEVP and H2b-emiRFP670

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    TEVP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)

    Plasmid Kit

    #1000000221

    Purpose

    A positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.

    Depositor

    Eric Cantor

    Available since

    June 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

    Plasmid

    #153005

    Purpose

    Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID-high affinity (C) (G79)
    

    Use

    Lentiviral

    Tags

    FRB, HA, and HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

    Plasmid

    #219548

    Purpose

    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

    Insert

    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The (SAGG)x4 linker between GAL4(1-65) and the PC…

    Promoter

    CMVd1

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397N

    Plasmid

    #189644

    Purpose

    16S rRNA Right_G1397N

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

    Insert

    TALE and DddAtox
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA

    Plasmid

    #115161

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.

    Inserts

    PTRE - tet activator responsive promoter
    flex sequence
    hM3dq-mCherry (inverted)
    flex sequence
    

    Use

    AAV

    Tags

    hM3Dq-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMEH303 NLS dTomato 6x high affinity MS2 loops

    Plasmid

    #168215

    Purpose

    Mammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loops

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    NLS dTomato
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397C_ABE

    Plasmid

    #189643

    Purpose

    16S rRNA Right_G1397C_ABE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

    Insert

    TALE and DddAtox and TadA 8e
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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