Addgene

We narrowed to 1,513 results for: SIR

Showing: 581 - 600 of 1513 results

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TagBFP_A1aY1

Plasmid

#158752

Purpose

Lentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

TagBFP-T_A1aY1

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

CAG (CMV enhancer and chicken beta actin promoter)

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTpPuc3

Plasmid

#62864

Purpose

Episome vector for Thalassiosira pseudonana (pUC-based)

Depositor

Hamilton Smith

Article

Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Episomal vector for thalassios…

Tags

Expression

Mutation

Promoter

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-UTRN-mini

Plasmid

#158749

Purpose

Expresses a shorter version of Utrophin (amino acid 35-136) with amino terminus eGFP in mammalian cells

Depositor

Minhajuddin Sirajuddin

Article

Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

Insert

UTRN-mini

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pRS426-TUB1-internal6xHis (1-440)

Plasmid

#60391

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4), amino a…

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB2a Cterminal tail

Plasmid

#60396

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pLV-Syn1-mCherry

Plasmid

#222967

Purpose

Make lentivirus to express mCherry in syn1 expressing cells

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

synapsin I

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-P19-EGFP

Plasmid

#196609

Purpose

siRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeat

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

p19 + EGFP inverted repeat sequence

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

AraC

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-P19-NP

Plasmid

#196608

Purpose

siRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeat

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

p19 + Influenza virus NP inverted repeat

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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LifeAct-14 (LA-14)

Plasmid

#158750

Purpose

Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.

Depositor

Minhajuddin Sirajuddin

Article

Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

Insert

LifeAct-14

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Deletion mutation for last three amino acids from…

Promoter

CMV immediate early

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2 - human TUBB6 Cterminal tail

Plasmid

#60403

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
p40651-EFSNS-FMR1_e1-24xms2

Plasmid

#222968

Purpose

MS2 mRNA reporter with intact FMR1 5' UTR and exon 1 sequence

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1-31CGG 5' UTR and exon1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a

Plasmid

#213401

Purpose

Plant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursor

Depositor

Alberto Carbonell

Article

Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:

Insert

Arabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2 - human TUBB1 Cterminal tail

Plasmid

#60400

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB3 Cterminal tail

Plasmid

#60397

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

C127S; TUB2 amino acids 429 - end, replaced with …

Promoter

GAL

Available since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB7 Cterminal tail

Plasmid

#60399

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta7 tubulin Cterminal tail (TUBB7) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB8 Cterminal tail

Plasmid

#60401

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta8 tubulin Cterminal tail (TUBB8) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB4a Cterminal tail

Plasmid

#60402

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
6xHis-TagRFP-T_A1aY1

Plasmid

#158754

Purpose

Expression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

6xHistidine_TagRFP-T_A1aY1

Use

Tags

6x-Histidine tag and TagRFP-T

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHR KIF17 (1-738)

Plasmid

#60406

Purpose

Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

KIF17 (KIF17 Human)

Use

Lentiviral

Tags

GB1, Strep-Tag, and sfGFP

Expression

Mammalian

Mutation

Truncated version amino acids 1-738

Promoter

SFFV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y

Plasmid

#60393

Purpose

Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Synthetic, Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4)

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only