We narrowed to 9,172 results for: mel

1. Hy_pMT Flag-Hel25E D193E

   Plasmid

   #110120

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   D193E mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMT-Pav-GFP

   Plasmid

   #24286

   Depositor

   Ron Vale

   Article

   Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

   Insert

   Pavarotti (pav Fly)
   

   Tags

   GFP

   Expression

   Insect

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pdU6-2_sgRNA1-d5-HT1BR

   Plasmid

   #221821

   Purpose

   Plasmid to express gRNA1 (gaaaatttgatttcaactga) for editing at the end of Drosophila 5-HT1BR coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   d5-HT1BR gRNA1 (5-HT1B Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   zebrafish U6 promoter (U6b)

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pdU6-2_sgRNA2-5-HT1BR

   Plasmid

   #221822

   Purpose

   Plasmid to express gRNA2 (aatttcgacgggccttcaag) for editing at the end of Drosophila 5-HT1BR coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   d5-HT1BR gRNA2 (5-HT1B Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   Drosophila U6 promoter

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Hy_pMT Flag-Hel25E MUT

   Plasmid

   #110122

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   4 mutations (KKLN motif changed to RSFS)

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Donor_C_FH_Nbr

   Plasmid

   #186662

   Purpose

   Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET45b-tsr(B/X) (6X-His-Dm-Cofilin)

   Plasmid

   #128278

   Purpose

   Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture

   Depositor

   Anna Sokac

   Article

   Figard et al Cell Rep. 2019 Mar 26;26(13):3493-3501.e

   Insert

   twinstar (tsr) (tsr Fly)
   

   Tags

   6X Histidine tag

   Expression

   Bacterial

   Mutation

   Met1 changed to Pro1; Cys77 silent basepair mutat…

   Promoter

   T7

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Donor_N_FH_Piwi

   Plasmid

   #186651

   Purpose

   Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a_DmKHC[1-421]-1xGFP-6xHis

   Plasmid

   #196972

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   6xHis and GFP

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PBAN-tetOn-NKX3.1

    Plasmid

    #238472

    Purpose

    Doxycycline inducible expression of the NKX3.1 transcription factor for mural cell differentiation.

    Depositor

    Juan Melero-Martin

    Article

    Lee et al Nat Commun. 2024 Sep 30;15(1):8392. doi:

    Insert

    NKX3.1 (NKX3-1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-2T-CBP(1603-2678)

    Plasmid

    #183774

    Purpose

    Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

    Insert

    nej (nej Fly)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMT-FLAG-CBPΔN

    Plasmid

    #182840

    Purpose

    A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2009 Sep;136(18):3131-41. d

    Insert

    CBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
    

    Tags

    FLAG tag

    Expression

    Insect

    Promoter

    MT

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

    Plasmid

    #196974

    Purpose

    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag

    Depositor

    Lukas Kapitein

    Article

    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Tags

    GST and mNeonGreen

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a_DmKHC[1-421]-SNAP-6xHis

    Plasmid

    #196975

    Purpose

    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

    Depositor

    Lukas Kapitein

    Article

    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Tags

    6xHis and SNAP

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KHBD00334

    Plasmid

    #34457

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG11491 (br Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pAc5.1B-EGFP-DmPenguin_B

    Plasmid

    #145924

    Purpose

    Insect Expression of DmPenguin

    Depositor

    Elisa Izaurralde

    Insert

    DmPenguin (peng Fly)
    

    Expression

    Insect

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CG4845/pUAST

    Plasmid

    #17588

    Depositor

    Denise Montell

    Insert

    CG4845 (psidin Fly)
    

    Expression

    Insect

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

18. CG4845/pUAST-HA

    Plasmid

    #17587

    Depositor

    Denise Montell

    Insert

    CG4845 (psidin Fly)
    

    Tags

    HA

    Expression

    Insect

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pAc5.1B-lambdaN-HA-DmRRP40_AA

    Plasmid

    #148221

    Purpose

    Insect Expression of DmRRP40

    Depositor

    Elisa Izaurralde

    Insert

    DmRRP40 (Rrp40 Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1C-Fluc-CG17271_A

    Plasmid

    #145864

    Purpose

    Insect Expression of CG17271-3UTR

    Depositor

    Elisa Izaurralde

    Insert

    CG17271-3UTR (CG17271 Fly)
    

    Expression

    Insect

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge