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Showing: 581 - 600 of 1319 results

  2. pTSara

    Plasmid
    #60720
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    N Terminal fragment of T7 RNAP split betwen 179-180
    N Terminal fragment of T7 RNAP split betwen 179-180
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T7 RNAP split between positions 179-180 and wt T7…
    Promoter
    PBAD
    Available since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60720-plasmid-map-sequence-id-97706

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  3. pSP019

    Plasmid
    #168195
    Purpose
    expresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.
    Depositor
    James Fleckenstein
    Insert
    eatA[H134R]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    H134R mutation
    Promoter
    araBAD
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    168195-plasmid-map-sequence-id-339468

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  4. pFG018

    Plasmid
    #62817
    Purpose
    Bacterial inducible gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    62817-plasmid-map-sequence-id-114422

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  5. pKP1469

    Plasmid
    #160815
    Purpose
    Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)
    Depositor
    Kathleen Postle
    Insert
    exbB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    160815-plasmid-map-sequence-id-316117

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  6. pX2-Cas9

    Plasmid
    #85811
    Purpose
    Arabinose inducible Cas9 in a broad host range backbone.
    Depositor
    Ryan Gill
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    85811-plasmid-map-sequence-id-177180

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  7. pCRT7 NT Topo arabinose

    Plasmid
    #73446
    Purpose
    Used for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptides
    Depositor
    Jesse Rinehart
    Type
    Empty backbone
    Use
    Tags
    GST (N-terminal), 6xHis (C-terminal)
    Expression
    Bacterial
    Mutation
    Promoter
    pBad
    Available since
    March 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    73446-plasmid-map-sequence-id-264560

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  8. pJaM

    Plasmid
    #67966
    Purpose
    Inducible TraJ, TraM expression with arabinose.
    Depositor
    Joshua Leonard
    Insert
    TraM, TraJ
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67966-plasmid-map-sequence-id-118466

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  9. pJ

    Plasmid
    #67965
    Purpose
    Indicuble TraJ expression with arabinose.
    Depositor
    Joshua Leonard
    Insert
    TraJ
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67965-plasmid-map-sequence-id-118469

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  10. CIDAR MoClo Parts Kit

    Plasmid Kit
    #1000000059
    Purpose
    CIDAR Modular Cloning (MoClo) kit uses modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in E. coli
    Depositor
    Douglas Densmore
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

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  12. pLaG2-T7RNAPC

    Plasmid
    #216337
    Purpose
    Arabinose-inducible PBad promoter driving expression of the anti-eGFP nanobody LaG2 translationally fused to the C-terminal portion (residues 180-883) of T7RNAP
    Depositor
    Mark Styczynski
    Insert
    LaG2-T7RNAPC fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    g2658a, g3915a
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    216337-plasmid-map-sequence-id-456751

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Showing: 581 - 600 of 1319 results