We narrowed to 9,887 results for: Coli
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Plasmid#157659PurposeGFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.DepositorInsertGFP-gRNA NatMX Z3EV
UseInsert storage (replicative in e. coli)Available SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Plas-CRISPR_reporter
Plasmid#157658PurposeCRISPR reporter for mRNA quantification, integrative plasmid into NPR2 geneDepositorInsertdCAS9-VP64, mCherry, KanMX
UseInsert storage (replicative in e. coli)MutationN28D in mCherry- please see depositor comments be…Available SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-LEU2
Plasmid#157656PurposeGFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequenceDepositorInsertGFP-gRNA
UseInsert storage (replicative in e. coli)TagsGFP-gRNAAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29b_1_anti-GFP-nanobody
Plasmid#210394PurposeExpress anti-GFP nanobody in E. coliDepositorInsert1_anti-GFP-nanobody
Tags6xHisExpressionBacterialAvailable SinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pG106 - KA
Plasmid#178041PurposeBacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, Kan and Amp selection markersDepositorTypeEmpty backboneUseShuttle vectorExpressionBacterialAvailable SinceFeb. 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCN38-yfp
Plasmid#227637PurposeNegative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.DepositorInsertyfp
ExpressionBacterialAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYHY2
Plasmid#216108PurposeConstitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.DepositorInsertNanoLuc
UseSynthetic Biology; E. coli-bacteroides shuttle ve…Tags3xFLAG tag and BT0294 signal peptideExpressionBacterialPromoterBfP1E6 promoterAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET16better_H10_HRV3C_chitinase
Plasmid#175091PurposeExpresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.DepositorInsertchitinase
Tags10X His, HRV-3CExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-30)
Plasmid#199167Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(-30)
TagsMGHHHHHHGGAMutationT19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJK225
Plasmid#71124PurposeExpression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coliDepositorInsertcitrate synthase
TagsHis6ExpressionBacterialPromoterT7Available SinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCB591
Plasmid#141096PurposeE. coli-Lactobacilli shuttle vector for recombineering template cloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI3
Plasmid#42527DepositorInsertWIPI3 (WDR45B Human)
TagsGST-TEVExpressionMammalianMutationgene sequence is codon optimized for expression i…Available SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCBFD1/HBFD1Bad
Plasmid#53364PurposeWill convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.DepositorInsertcbfd1
ExpressionBacterialPromoterlacAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S2
Plasmid#128590PurposeExpresses tagless S2 ribosomal protein in E.coliDepositorAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIA1414
Plasmid#172402PurposeExpression of SARS-COV-2 Nsp9 in E. coliDepositorAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPdR
Plasmid#85083PurposeExpresses putidaredoxin reductase in E. coliDepositorInsertPutidaredoxin reductase
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
His6-SUMO-pET28a-EfaCas1
Plasmid#112793PurposeTo express enterococcus faecalis Cas1 protein in E. coliDepositorInsertEfaCas1
TagsHis6-SUMOExpressionBacterialAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
PARG-c002
Plasmid#173107PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACλCI-β-flap (831-1057)
Plasmid#53733PurposeExpression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertλCI-β-flap fusion
ExpressionBacterialPromoterlac UV5Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBRα-β flap (831-1057)
Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertalpha NTD-Beta flap fusion
ExpressionBacterialPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only