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mCerulean3-EB3-C-20

Plasmid

#55417

Purpose

Localization: MT End Binding Protein, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

EB3 (MAPRE3 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
PHR-GFP

Plasmid

#183926

Purpose

Optogenetic PHR domain coupled to GFP; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP (CRY2 Mustard Weed, Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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mOrange2-Vimentin-7

Plasmid

#57977

Purpose

Localization: Intermediate Filaments, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Vimentin (VIM Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
IBMc326

Plasmid

#161949

Purpose

Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter

Expression

Bacterial

Promoter

PBAD

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQTEV-AK1

Plasmid

#31290

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

adenylate kinase 1 (AK1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pLV.U6gT28.13.EFS-NS.H2B-RFP

Plasmid

#170388

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-223-Decoy.hPGK.GFP.WPRE

Plasmid

#46601

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-223 Decoy (MIR223, Mir223 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I

Plasmid

#146553

Purpose

Mammalian Expression of HsSmg6-del36-576-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-del36-576-siRNAres (SMG6 Human)

Expression

Mammalian

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_TLOC1 (220aa)

Plasmid

#45273

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TLOC1 (SEC62 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pMiniT:myc-GluA1 TKIT donor

Plasmid

#169419

Purpose

Donor DNA for TKIT knockin of Myc to GluA1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Myc (Myc Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Vimentin-7

Plasmid

#55452

Purpose

Localization: Intermediate Filaments, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Vimentin (VIM Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P41nmt1-GST

Plasmid

#39287

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin

Plasmid

#134866

Purpose

Expresses EGFP-tagged mutant of VAMP1

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Vesicle-associated membrane protein 1 (VAMP1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 97-118

Promoter

EF1a

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-146a-Decoy.hPGK.GFP.WPRE

Plasmid

#46590

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-146a Decoy (MIR146A, Mir146 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

Plasmid

#160610

Purpose

Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

P35s:AsCas12a:T35s

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

Plasmid

#182354

Purpose

Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

Plasmid

#182356

Purpose

Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAT-9222

Plasmid

#124222

Purpose

Cloning plasmid for EGxFP assay with self-targeting gRNA-s

Depositor

Ervin Welker

Article

Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

Insert

self-targeting gRNA between EGFP halves

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFL His-SDS22

Plasmid

#113512

Purpose

Expression of His-SDS22 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

SDS22 (PPP1R7 Human)

Tags

6*His

Expression

Insect

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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hs-lambda btl

Plasmid

#17581

Depositor

Denise Montell

Article

Lee et al Genes Dev. 1996 Nov 15. 10(22):2912-21.

Insert

lambda-btl (btl Fly)

Expression

Insect

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only