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mTB3-AviD tag

Plasmid

#210203

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the AviD-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Publication

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

AviD-tag

Expression

Bacterial

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B3-HA

Plasmid

#209228

Purpose

Mammalian expression of ATP8B3

Depositor

Hye-Won Shin

Publication

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B3 (ATP8B3 Human)

Use

Retroviral

Tags

HA

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag6-iCAM-1

Plasmid

#205210

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG6

Depositor

Wendell Lim

Publication

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag6 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag18-iCAM-1

Plasmid

#205207

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG18

Depositor

Wendell Lim

Publication

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag18 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag42-iCAM-1

Plasmid

#205205

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG42

Depositor

Wendell Lim

Publication

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag42 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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PMT-mEGFP_2

Plasmid

#203778

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Publication

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mEGFP dimer

Expression

Mammalian

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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PMT-mTurquoise2_2

Plasmid

#203776

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Publication

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mTurquoise2 dimer

Expression

Mammalian

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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PMT-mCherry_2

Plasmid

#203782

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Publication

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mCherry dimer

Expression

Mammalian

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2- PIP4K2CD280K

Plasmid

#202731

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2C (D280K) (PIP4K2C Human)

Expression

Mammalian

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP-MOOR

Plasmid

#198212

Purpose

pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tag

Depositor

Michael Jewett

Publication

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

sfGFP_MOOR

Tags

6x His Tag and MOOR glycosylation tag

Expression

Bacterial

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pYFAC-CH1

Plasmid

#168977

Purpose

AMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacI

Depositor

Yit Heng Chooi

Publication

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in fil…

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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C413-E38: attB4-T7p>-attB1r

Plasmid

#162929

Purpose

Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

bacteriophage T7 promoter

Use

Synthetic Biology

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E25: attB2r-SNAP-attB3

Plasmid

#162914

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal SNAP tagged fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SNAP tag

Use

Synthetic Biology

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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PsaA

Plasmid

#159997

Purpose

Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

Depositor

Mark Howarth

Publication

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

PsaA (19-309)

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Spy0469

Plasmid

#159996

Purpose

Expresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cells

Depositor

Mark Howarth

Publication

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

Spy0469 (24-389)

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 mTFP1 CTAG

Plasmid

#153400

Purpose

Cyan fluorescent protein with FLAG tag

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

mTFP1 CTAG

Tags

FLAG tag

Expression

Plant

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pXF262

Plasmid

#139038

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

cox-4 (no STOP codon) (W09C5.8 Nematode)

Expression

Worm

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSM05

Plasmid

#139050

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mkate2 (includes STOP codon)

Expression

Worm

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSDH66

Plasmid

#139053

Purpose

MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

unc-54 3'UTR (unc-54 Nematode)

Expression

Worm

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK103

Plasmid

#110582

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Lac promoter with lac operator and RBS

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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