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pCS1

Plasmid

#55749

Purpose

Produces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Expression

Bacterial

Promoter

T7-lac

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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VV228: sdChR(C138S E154A)-TS in fck

Plasmid

#58523

Purpose

Expresses sdChR(C138S E154A) in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S, E154A)-TS

Use

Lentiviral

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine; changed Glutamate…

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Snail1-3'UTR

Plasmid

#61781

Purpose

Luciferase reporter containing the 3'UTR of mouse Snail1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Snail1 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pBGW

Plasmid

#62665

Purpose

Donor vector for mCitrine knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

mCitrine

Use

Cre/Lox

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBEZ

Plasmid

#62669

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

tdTomato

Use

Cre/Lox

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBFB

Plasmid

#62668

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

tdTomato

Use

Cre/Lox

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 L1-R5

Plasmid

#62111

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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AU1-TTC30B-pcDNA3

Plasmid

#47325

Purpose

Expresses AU1 epitope-tagged TTC30B in mammalian cells

Depositor

Richard Maurer

Article

Howard et al Exp Cell Res. 2013 Jun 25. pii: S0014-48

Insert

TTC30B (Ttc30b Mouse)

Tags

AU1 epitope

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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mGDF8 donor mCherry-PA

Plasmid

#72596

Purpose

Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

mCherry-puromycin cassetter

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
p3E-mCherry-FRB-HA no-pA

Plasmid

#82595

Purpose

3' entry vector containing HA epitope fused to C-terminus and mCherry fused to N-terminus of FRB without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mCherry-FRB-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMCh-56

Plasmid

#92260

Purpose

Plasmid for expression of HA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

Depositor

Marina Chekulaeva, Roy Parker

Article

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 (gw Fly)

Tags

HA

Expression

Insect

Promoter

Ac5

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS3-IVS-Syn21-GFP-P10

Plasmid

#104614

Purpose

Expresses GFP upon binding of TALE3 to VAS3

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS3

Expression

Insect

Promoter

hsp70

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS1-IVS-Syn21-GFP-P10

Plasmid

#104613

Purpose

Expresses GFP upon binding of TALE1 to VAS1

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS1

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE1-VP64-P10

Plasmid

#104606

Purpose

Expresses TALE1 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE1

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBW811_pCAG-PV8-F14-GFP-T2A-mCherry-BGHpA

Plasmid

#87587

Purpose

PV8 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP, mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D2 LxCxE HA

Plasmid

#8957

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D2 (CCND2 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of LxCxE motif

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
AU1-TTC30A2-pcDNA3

Plasmid

#47324

Purpose

Expresses AU1 epitope-tagged TTC30A2 in mammalian cells

Depositor

Richard Maurer

Article

Howard et al Exp Cell Res. 2013 Jun 25. pii: S0014-48

Insert

TTC30A2 (Ttc30a2 Mouse)

Tags

AU1 epitope

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR H1 L5-L4

Plasmid

#62102

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 L171C

Plasmid

#66177

Purpose

Expresses the L171C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Mutation

L171C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 G108C

Plasmid

#66140

Purpose

Expresses the G108C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Mutation

G108C

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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