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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 60,235 results for: cloning

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Showing: 6061 - 6080 of 60235 results

  1. KRASB

    Plasmid
    #25153
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KRASb:M1-K169 (KRAS Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Q61H
    Available Since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pLCS111

    Plasmid
    #209332
    Purpose
    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal mScarlet-I-mNeonGreen tandem fluorescent timer fusion protein.
    Depositor
    Lucia Strader
    Article
    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209332-plasmid-map-sequence-id-420447

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  7. pCZGY2850

    Plasmid
    #87758
    Purpose
    Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.
    Depositor
    Andrew Chisholm
    Article
    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10
    Insert
    Pcol-19-PH-miniSOG(Q103L)
    Tags
    PH
    Expression
    Worm
    Mutation
    Q103L
    Promoter
    Pcol-19
    Available Since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    87758-plasmid-map-sequence-id-174819

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  9. pFD115

    Plasmid
    #124768
    Purpose
    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    Use
    CRISPR
    Promoter
    Ptet
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124768-plasmid-map-sequence-id-246185

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  14. pAGD289

    Plasmid
    #228113
    Purpose
    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Kva1 retron, ncRNA with 90 bp rpoB donor
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228113-plasmid-map-sequence-id-455374

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  19. p663-UBC-miniTurbo-V5-NEK1_IDG-K

    Plasmid
    #189871
    Purpose
    Gateway destination clone of NEK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK1 (NEK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    189871-plasmid-map-sequence-id-374462

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Showing: 6061 - 6080 of 60235 results