We narrowed to 59,538 results for: cloning

1. pMC-X4

   Plasmid

   #176154

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMC-XII1

   Plasmid

   #176159

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMC-XI3

   Plasmid

   #176157

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. p005kanRFP

   Plasmid

   #58533

   Purpose

   RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   Red fluorescent protein
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T5 between two lac operators

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMC-XII5

   Plasmid

   #176161

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL50015

   Plasmid

   #50318

   Purpose

   Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, mNEON (Branchistoma lanceolatum)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFH113

   Plasmid

   #128210

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-TagRFPt-NS

   Plasmid

   #75343

   Purpose

   Multisite gateway entry clone for adding N-terminal fusions of TagRFPt

   Depositor

   Rob Parton

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   TagRFPt
   

   Expression

   Mammalian

   Available Since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-Kan_BR29_00091361

   Plasmid

   #121209

   Purpose

   Level 0 Golden-Gate compatible vector

   Depositor

   Sophien Kamoun

   Article

   Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

   Insert

   BR29_00091361
   

   Use

   Golden gate

   Mutation

   Magnaporthe grisea

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJC225

    Plasmid

    #176883

    Purpose

    MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Cloning

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p663-UBC-miniTurbo-V5-ERN2_IDG-K

    Plasmid

    #189905

    Purpose

    Gateway destination clone of ERN2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ERN2 (ERN2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJRA103

    Plasmid

    #209336

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal SNAP-tag fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-nlsGal80(opt)

    Plasmid

    #51618

    Purpose

    vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

    Depositor

    Joshua Bonkowsky

    Article

    Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

    Insert

    Gal80(opt) (GAL80 Budding Yeast)
    

    Use

    Vertebrate expression

    Tags

    NLS

    Mutation

    codon optimized

    Available Since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pF hPGK mc2 IRES neo

    Plasmid

    #206236

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.23-SCP1-ccdB

    Plasmid

    #131628

    Purpose

    Modified STARR-seq plasmid to measure enhancer activity (SCP1 promoter; ccdb gene at cloning site)

    Depositor

    Kevin White

    Article

    Liu et al Genome Biol. 2017 Nov 20;18(1):219. doi:

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Bacterial and Mammalian

    Promoter

    SCP1

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUPD-Cyc1t (17-21)

    Plasmid

    #101700

    Purpose

    Cyc1t in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    GCTT-[terminator of S. cerevisiae YJR048W (190 bp)]-CGCT
    

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUPD-pTEF2 (01-11)

    Plasmid

    #101685

    Purpose

    pTEF2 in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    GGAG-[promoter of S. cerevisiae YBR118W (520bp)]-CCAT
    

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFH49

    Plasmid

    #128211

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBBR5pemIK-GW

    Plasmid

    #126556

    Purpose

    Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatible

    Depositor

    Lindsey Burbank, Drake Stenger

    Article

    Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pL0M-S-mClover3-EC18157

    Plasmid

    #137076

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mClover3 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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