We narrowed to 14,429 results for: nts

1. pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10

   Plasmid

   #197965

   Purpose

   T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pco-nCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26 and pUBQ10

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEE495

   Plasmid

   #149291

   Purpose

   T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmalc4x_I499F_TEV

   Plasmid

   #47703

   Purpose

   Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

   Depositor

   Raquel Lieberman

   Article

   Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

   Insert

   Olfactomedin-like domain with I499F mutation (MYOC Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed I499F

   Promoter

   Ptac

   Available since

   Sept. 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pUPD2 AtUBQ10 (GB2478)

   Plasmid

   #160594

   Purpose

   UBQ10 promoter from A. thaliana for constitutive expression in plant systems.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AtUBQ10
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSADdeltaG-GFP-AlstR

   Plasmid

   #32647

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Inserts

   GFP
   AlstR (AstA-R1 Fly)
   

   Use

   Rabies virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pT7-SpCas9_sgRNA-site2 (RTW448)

   Plasmid

   #160137

   Purpose

   T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPO636

   Plasmid

   #195299

   Purpose

   pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   TnsABCQ operon of McCAST (Tn7575)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   IPTG inducible lac promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PKG I beta CAT

   Plasmid

   #16396

   Depositor

   Bernard Weinstein

   Article

   Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

   Insert

   PKG1 beta (PRKG1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   CAT: catalytic domain

   Promoter

   Available since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.2 mir-1-1 reporter hsa-let-7a-1

   Plasmid

   #46670

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-let-7a-1 (MIRLET7A1 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB526

    Plasmid

    #74868

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDECKO_Malat1_E

    Plasmid

    #72624

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD-BirA-eCPX-AP(K10)

    Plasmid

    #121908

    Purpose

    Expresses BirA and eCPX-AP(K10)

    Depositor

    Per Svenningsen

    Article

    Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

    Inserts

    BirA (birA Synthetic)
    enhanced Circularly Permuted Outer Membrane Protein X
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed lysine 10 in the AP to alanine

    Promoter

    T7 and pBAD promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMOD_A0608

    Plasmid

    #91018

    Purpose

    Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9_D10A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EC1.2

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGE663

    Plasmid

    #153261

    Purpose

    M6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGE665

    Plasmid

    #153263

    Purpose

    M8E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE654

    Plasmid

    #153252

    Purpose

    M1E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSETa-Amrose v3

    Plasmid

    #79539

    Purpose

    pRSETa (Invitrogen) plasmid expressing Amrose variant 3

    Depositor

    Randolph Caldwell

    Article

    Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

    Insert

    Amrose v3
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 3xFLAG TPP1 88-249

    Plasmid

    #53549

    Purpose

    mammalian expression of TPP1 OB-fold domain

    Depositor

    Kathleen Collins

    Article

    Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

    Insert

    TPP1 (ACD Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    aa 88-249

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

    Plasmid

    #192681

    Purpose

    Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMOD_A1710

    Plasmid

    #91039

    Purpose

    Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-TaCas9_H840A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    H840A

    Promoter

    ZmUbi

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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