We narrowed to 7,744 results for: sms

1. PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA

   Plasmid

   #176854

   Purpose

   Expresses membrane bound biotin ligase, BioID2, in neurons

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Lck-13xLinker-BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   hSyn1

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas12b(RuvC)

   Plasmid

   #186450

   Purpose

   Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   dCas12b
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   E848A, D977A

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mKate2-P2A-APEX2-OSBP

   Plasmid

   #67664

   Purpose

   Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-OSBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PZac2.1 hSyn1-HA-BioID2-SAPAP3

   Plasmid

   #176857

   Purpose

   Expresses SAPAP3 protein fused with BioID2 at the N-terminus in neurons

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   HA-BioID2-SAPAP3
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   hSyn1

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-Tagger

   Plasmid

   #129396

   Purpose

   Expresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoter

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS Biol. 2019 Aug 8;17(8):e3000374. do

   Insert

   rpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
   

   Use

   Tags

   FLAG, HA, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF-ManII-GFP

   Plasmid

   #160905

   Purpose

   Expresses the Golgi marker ManII-GFP in mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   MAN2A1 (MAN2A1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

   Plasmid

   #159283

   Purpose

   A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Inserts

   Human INS sgRNA
   Human INS guide RNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. miniSOG-H2B-C-10

   Plasmid

   #57769

   Purpose

   Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. myristoylated ct-GRK2

   Plasmid

   #178734

   Purpose

   N-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)

   Depositor

   Ege Kavalali

   Article

   Alten et al Cell Rep. 2022 Jan 18;38(3):110255. doi:

   Insert

   GRK2 (GRK2 Bovine)
   

   Use

   Lentiviral

   Tags

   Src 1-15

   Expression

   Mutation

   only amino acids 548-671

   Promoter

   Ubiquitin

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mKate2-P2A-APEX2-ML1N*2

    Plasmid

    #67665

    Purpose

    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-ML1N*2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FLAG-HA-DGCR8

    Plasmid

    #106251

    Purpose

    Express DGCR8 in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Chang et al Genome Res. 2015 Sep;25(9):1401-9. doi:

    Insert

    DiGeorge Syndrome Critical Region 8 (DGCR8 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNeonGreen-C1-Nir2-LNS2

    Plasmid

    #220086

    Purpose

    C-terminal PA binding domain of Nir2

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PITPNM1 (PITPNM1 Human)
    

    Use

    Tags

    NeonGreen-GGSGGM

    Expression

    Mammalian

    Mutation

    amino acids 896-1216

    Promoter

    CMV

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA

    Plasmid

    #176745

    Purpose

    Expresses Glt-1 fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Glt1-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR SFFVp MCP-mCherry IRES h2b-diRFP

    Plasmid

    #102351

    Purpose

    Lentiral expression vector bearing the MS2 Coat Protein (MCP) fused to mCherry as well as the nuclear marker histone 2b fused to two copies of irFP. Also bears the hygromycin selection marker.

    Depositor

    Jared Toettcher

    Article

    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

    Insert

    MS2 Coat Protein
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-7aa-ActA-pEGFP-C1

    Plasmid

    #177406

    Purpose

    To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.

    Depositor

    David Andrews

    Article

    Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

    Insert

    ActA tail anchor (actA Listeria monocytogenes)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMG-miR-125b-1

    Plasmid

    #58992

    Purpose

    MSCV based expression vector - co-expresses GFP and human miR-125b-1

    Depositor

    David Baltimore

    Article

    So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

    Insert

    miR-125b-1 (MIR125B1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA

    Plasmid

    #176743

    Purpose

    Expresses Connexin-43 fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Cx43-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

    Plasmid

    #67668

    Purpose

    Zebrafish expression vector for ubiquitous expression of APEX2-GBP

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Use

    Zebrafish expression with tol2

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNES-EGFP-C1-flex-PABD-Spo20

    Plasmid

    #220081

    Purpose

    Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    Spo20 (SPO20 Budding Yeast)
    

    Use

    Tags

    X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

    Expression

    Mammalian

    Mutation

    amino acids 51-91

    Promoter

    CMV

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFlox_N-Mau2_Neo_FKBPF36V_mScarlet

    Plasmid

    #163881

    Purpose

    Acute Depletion of Mau2-mScarlet

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    Mau2 (Mau2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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