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We narrowed to 8,222 results for: mme

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Showing: 6081 - 6100 of 8222 results

  2. pCMV CHMP2Bchimera-L4D,F5D-CHMP6α2-3XHA

    Plasmid
    #242419
    Purpose
    Expression of a CHMP2B membrane binding mutant (L4D F5D) with the second alpha helix (residues 55-96) replaced by that from CHMP6 (residues 57-98) and a 3xHA tag.
    Depositor
    Franz-Ulrich Hartl
    Article
    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e
    Inserts
    CHMP2B (CHMP2B Human)
    CHMP6 (CHMP6 Human)
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    L4D F5D; Helix 2 (residues 55-96) replaced with r…
    Promoter
    CMV
    Available Since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    242419-plasmid-map-sequence-id-500686

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  6. pPHR-mCherry-RGG-GFP-RGG-mHP1alpha

    Plasmid
    #181893
    Purpose
    Soluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-RGG-GFP-RGG-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181893-plasmid-map-sequence-id-359709

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  7. pPHR-mCherry-nE-mHP1alpha-delCTE

    Plasmid
    #181891
    Purpose
    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mouse, Mustard Weed)
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion
    Promoter
    CMV
    Available Since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181891-plasmid-map-sequence-id-359705

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  8. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid
    #213031
    Purpose
    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration
    Depositor
    Donna Romero, Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    Use
    Luciferase
    Tags
    Firefly luciferase antisense intron (3' UTR)
    Expression
    Mammalian
    Promoter
    pTRE bidirectional tet-on
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213031-plasmid-map-sequence-id-422025

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  9. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  11. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213030-plasmid-map-sequence-id-422062

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  12. Bxb1-GA_AIO-TetOn-Ngn2_TetOn_ISL1-LHX3_Divergent

    Plasmid
    #250433
    Purpose
    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of Ngn2, ISL1 and LHX3
    Depositor
    Richard Davis, Kate Galloway
    Article
    Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103
    Inserts
    Ngn2 (NEUROG2 Human)
    ISL1-T2A-LHX3 (ISL1, LHX3 Human)
    TetOn3G rtTA
    Tags
    3xHA and T2A-mTagBFP2-3xNLS
    Expression
    Mammalian
    Promoter
    CAG and TRE3G
    Available Since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    250433-plasmid-map-sequence-id-497778

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  13. Split 'n coexpress CUL3-RBX1

    Plasmid
    #52293
    Purpose
    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1
    Depositor
    Brenda Schulman
    Article
    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1
    Inserts
    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    Tags
    GST, IRES, and TEV
    Expression
    Bacterial
    Mutation
    contains aa 1-309, contains aa 310-768, and conta…
    Promoter
    IRES and tac
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. Bxb1-GA_AIO-TetOn_Ngn1_TetOn_ISL1_TetOn_LHX3_Down-Tandem

    Plasmid
    #250432
    Purpose
    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of Ngn2, ISL1 and LHX3
    Depositor
    Richard Davis, Kate Galloway
    Article
    Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103
    Inserts
    Ngn2 (NEUROG2 Human)
    ISL1 (ISL1 Human)
    LHX3 (LHX3 Human)
    TetOn3G rtTA
    Tags
    3xHA and T2A-mTagBFP2-3xNLS
    Expression
    Mammalian
    Promoter
    CAG and TRE3G
    Available Since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    250432-plasmid-map-sequence-id-497777

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  16. Bxb1-GT-Dox-AIO-TetOn_Ngn2-ISL1-LHX3_Down-Tandem

    Plasmid
    #241396
    Purpose
    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of Ngn2, ISL1 and LHX3
    Depositor
    Richard Davis
    Article
    Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103
    Insert
    TRE-Ngn2-ISL1-LHX3; rtTA-T2A-mTagBFP2 (ISL1, LHX3, NEUROG2 Human)
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    TRE; CAG
    Available Since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    241396-plasmid-map-sequence-id-489344

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  17. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213029
    Purpose
    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213029-plasmid-map-sequence-id-422050

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  18. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  19. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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  20. Bxb1-GA-Dox-AIO-TetOn_Ngn2-ISL1-LHX3_Down-Tandem

    Plasmid
    #250431
    Purpose
    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of Ngn2, ISL1 and LHX3
    Depositor
    Richard Davis, Kate Galloway
    Article
    Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103
    Inserts
    Ngn2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)
    TetOn3G rtTA
    Tags
    T2A-mTagBFP2-3xNLS
    Expression
    Mammalian
    Promoter
    CAG and TRE3G
    Available Since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    250431-plasmid-map-sequence-id-497784

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Showing: 6081 - 6100 of 8222 results