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pET28a_6HT-TBP6.7(R52A)

Plasmid

#112729

Purpose

52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R52A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R52A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(delta C-term)

Plasmid

#112731

Purpose

This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(delta C-term)

Tags

6His-TEV

Expression

Bacterial

Mutation

delta C-term

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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CRY-GalVP16 (B695)

Plasmid

#92031

Purpose

Expresses CRY2 (full length, intact NLS) fusion with Gal4DBD(aa1-147) fused to VP16AD (truncated), downstream of mCherry-IRES.

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Tags

GalBD-VP16AD

Expression

Mammalian

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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Tpa1(273-644)

Plasmid

#23141

Depositor

SeWon Suh

Article

Kim et al Nucleic Acids Res. 2010 Apr;38(6):2099-1

Insert

Tpa1 C-terminal domain (TPA1 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-272

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pRB117.2

Plasmid

#181949

Purpose

aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

mNG-NarL; mCherry; tetR (narL, AX04_RS23485 Synthetic, narL - E. coli)

Tags

mNeonGreen

Expression

Bacterial

Mutation

See Depositor Comments Below

Promoter

mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRB113.2

Plasmid

#181948

Purpose

aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

NarL-mNG; mCherry; tetR (AX04_RS23485, narL Synthetic, narL - E. coli)

Tags

mNeonGreen

Expression

Bacterial

Promoter

NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRB117.2

Plasmid

#181949

Purpose

aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

mNG-NarL; mCherry; tetR (narL, AX04_RS23485 Synthetic, narL - E. coli)

Tags

mNeonGreen

Expression

Bacterial

Mutation

See Depositor Comments Below

Promoter

mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRB113.2

Plasmid

#181948

Purpose

aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

NarL-mNG; mCherry; tetR (AX04_RS23485, narL Synthetic, narL - E. coli)

Tags

mNeonGreen

Expression

Bacterial

Promoter

NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKD317.8

Plasmid

#181951

Purpose

IPTG-inducible expression of NarX

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

NarX; lacI (narX, lacI NarX - E. coli; lacI - E. coli)

Expression

Bacterial

Promoter

NarX-Ptac; lacI-PlacIq

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKD317.8

Plasmid

#181951

Purpose

IPTG-inducible expression of NarX

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

NarX; lacI (narX, lacI NarX - E. coli; lacI - E. coli)

Expression

Bacterial

Promoter

NarX-Ptac; lacI-PlacIq

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJ

Plasmid

#67965

Purpose

Indicuble TraJ expression with arabinose.

Depositor

Joshua Leonard

Insert

TraJ

Use

Synthetic Biology

Tags

FLAG

Promoter

pBAD

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJ

Plasmid

#67965

Purpose

Indicuble TraJ expression with arabinose.

Depositor

Joshua Leonard

Insert

TraJ

Use

Synthetic Biology

Tags

FLAG

Promoter

pBAD

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJaM

Plasmid

#67966

Purpose

Inducible TraJ, TraM expression with arabinose.

Depositor

Joshua Leonard

Insert

TraM, TraJ

Use

Synthetic Biology

Promoter

pBAD

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJaM

Plasmid

#67966

Purpose

Inducible TraJ, TraM expression with arabinose.

Depositor

Joshua Leonard

Insert

TraM, TraJ

Use

Synthetic Biology

Promoter

pBAD

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pShuttle

Plasmid

#67964

Purpose

Mobilizible plasmid with EGFP. OriT from the F plasmid.

Depositor

Joshua Leonard

Insert

OriT

Use

Synthetic Biology

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pShuttle

Plasmid

#67964

Purpose

Mobilizible plasmid with EGFP. OriT from the F plasmid.

Depositor

Joshua Leonard

Insert

OriT

Use

Synthetic Biology

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLNKR1

Plasmid

#67968

Purpose

High copy number plasmid encoding part of the tra Operon PY-traR

Depositor

Joshua Leonard

Insert

tra Operon (First Quarter)

Use

Synthetic Biology

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLNKR1

Plasmid

#67968

Purpose

High copy number plasmid encoding part of the tra Operon PY-traR

Depositor

Joshua Leonard

Insert

tra Operon (First Quarter)

Use

Synthetic Biology

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLNKR2

Plasmid

#67969

Purpose

High copy number plasmid encoding part of the tra Operon traC-artA

Depositor

Joshua Leonard

Insert

tra Operon (second Quarter)

Use

Synthetic Biology

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLNKR2

Plasmid

#67969

Purpose

High copy number plasmid encoding part of the tra Operon traC-artA

Depositor

Joshua Leonard

Insert

tra Operon (second Quarter)

Use

Synthetic Biology

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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