We narrowed to 11,952 results for: GFP expression plasmids

1. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

   Plasmid

   #85756

   Purpose

   All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Inserts

   Csy4-T2A-SpRFN-P2A-GFP
   gRNA
   

   Use

   CRISPR

   Tags

   Csy4, Csy4 recognition sequence, FokI fusion via …

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CMV and U6

   Available Since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV uN2C GFP (Ctl)

   Plasmid

   #224355

   Purpose

   Express GFP tagged upsteram ORF of NOTCH2NLC with a control size (12x) of GGC repeats

   Depositor

   Nicolas Charlet-Berguerand

   Article

   Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

   Insert

   uN2C
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Codon-optimized for expression in human, so no pu…

   Promoter

   CMV + chimeric intyron (CAG)

   Available Since

   Oct. 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pZ M2rtTA_CAGG TetON-Sox10 with GFP

   Plasmid

   #115241

   Purpose

   RMCE donor vector for inducible expression of SOX10 and GFP and constitutive expression of m2rtTA (generated from plasmid #112668)

   Depositor

   Catherine Verfaillie

   Article

   Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

   Inserts

   SOX10 (SOX10 Human)
   eGFP
   

   Tags

   GFP (separate polypeptide)

   Expression

   Mammalian

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMR506-EF1a-H2B-EGFP

   Plasmid

   #186627

   Purpose

   Expresses nuclear-localised EGFP under EF1a promoter. For insertion of genetic barcodes into 3'UTR of EGFP.

   Depositor

   Jonas Frisén

   Article

   Ratz et al Nat Neurosci. 2022 Mar;25(3):285-294. do

   Insert

   EF1a (EEF1A1 Synthetic)
   

   Use

   Lentiviral

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCherryGFP-CoV2-Inframe-stop

   Plasmid

   #177620

   Purpose

   Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcBac3-EcTrp-TGA-EGFP**

   Plasmid

   #228786

   Purpose

   EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTrpRS
   EcWtR
   EGFP**
   

   Use

   Baculoviral

   Expression

   Mammalian

   Mutation

   TGA suppressor

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-H1-shSRGAP2B/C#2-hsynapsin-EGFP

   Plasmid

   #220797

   Purpose

   shRNA plasmid against human SRGAP2B/C genes (#2) with EGFP expression in neurons

   Depositor

   Pierre Vanderhaeghen

   Article

   Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

   Insert

   EGFP
   

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pS34.EF1α<GFP-2A-Cas9-RC

   Plasmid

   #207404

   Purpose

   Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   Cas9-RC
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GFP-2A

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. attB-GFP-Dnmt3b1-Poly(A)

   Plasmid

   #65531

   Purpose

   Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   isoform 1

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-H1-shSRGAP2A-hsynapsin-EGFP

    Plasmid

    #220798

    Purpose

    shRNA plasmid against human SRGAP2A gene with EGFP expression in neurons

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

    Insert

    EGFP
    

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACT1:Cas9-GFP, U6:sgTK

    Plasmid

    #122852

    Purpose

    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Inserts

    Cas9-GFP
    U6-sgTK
    

    Use

    CRISPR; Cas9-gfp plasmid for genome editing of cr…

    Tags

    eGFP

    Promoter

    Cryptosporidium parvum U6 and Cryptosporidium par…

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACT1:Cas9-GFP, U6:sgUPRT

    Plasmid

    #122853

    Purpose

    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Inserts

    Cas9-GFP
    U6-sgUPRT
    

    Use

    CRISPR; Cas9-gfp plasmid for genome editing of cr…

    Tags

    eGFP

    Promoter

    Cryptosporidium parvum U6 and Cryptosporidium par…

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSAD-5PSD95-EGFP-SynPhRFP

    Plasmid

    #217979

    Purpose

    G-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP

    Depositor

    Edward Callaway

    Article

    Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

    Inserts

    5PSD95-EGFP (Dlg4 Rat)
    SynPhRFP (Syp Mouse)
    

    Use

    G-deleted rabies

    Tags

    EGFP and RFP

    Promoter

    None

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT2/GD-IRES-GFP-CTNNB1

    Plasmid

    #192868

    Purpose

    Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

    Depositor

    David Largaespada

    Article

    Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

    Inserts

    luc+
    EGFP
    CTNNB1
    

    Expression

    Mammalian

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. attB-GFP-Tet1-Poly(A)

    Plasmid

    #65559

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locus

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. attB-GFP-Dnmt3b6-Poly(A)

    Plasmid

    #65532

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    isoform 6

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-shCasp2-IRES-GFP

    Plasmid

    #52061

    Purpose

    stable knockdown of Caspase-2 in mammalian cells

    Depositor

    Tyler Jacks, Trudy Oliver

    Article

    Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

    Insert

    Caspase-2 shRNA (CASP2 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

    Plasmid

    #85821

    Purpose

    iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    Zf Ubi::cMyc oDam_f_GFPoNLS
    

    Use

    Tol2 based vector

    Tags

    cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-H1-shSRGAP2B/C#1-hsynapsin-EGFP

    Plasmid

    #220796

    Purpose

    shRNA plasmid against human SRGAP2B/C genes (#1) with EGFP expression in neurons

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

    Insert

    EGFP
    

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEos2-dVHH[GFP] in pCSDEST

    Plasmid

    #162870

    Purpose

    Super-resolution of GFP in culture

    Depositor

    Rob Parton

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    mEos2-dVHH[EGFP]
    

    Tags

    mEos2

    Expression

    Mammalian

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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