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We narrowed to 6,862 results for: GFP expression plasmids

Showing: 6101 - 6120 of 6862 results

  1. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid
    #131312
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    131312-plasmid-map-sequence-id-254654

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  9. pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

    Plasmid
    #190236
    Purpose
    AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase
    Depositor
    Hiroyuki Hioki
    Article
    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi
    Insert
    EGFP
    Use
    AAV
    Tags
    F2A, LDLR C-terminal sequence, mRFP1, myristoylat…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    190236-plasmid-map-sequence-id-381571

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  11. PX458-3xHA-en31FnCas9

    Plasmid
    #201949
    Purpose
    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    3xHA-NLS-en31FnCas9-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    G1243T, E1369R, E1603H on FnCas9
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201949-plasmid-map-sequence-id-405303

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  14. PX458-3xHA-FnCas9ABEmax8.17d

    Plasmid
    #201955
    Purpose
    Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D11A on FnCas9, V82S, V106W, Q154R on mutant TadA
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201955-plasmid-map-sequence-id-405309

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  15. pNoROS-CAT

    Plasmid
    #170152
    Purpose
    Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation
    Depositor
    Dimitri Pestov
    Article
    Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.
    Inserts
    catalase (Cat Mouse)
    roGFP2
    Use
    Genome integration via sleeping beauty transposon…
    Tags
    FLAG tag and Nucleolar Localization Sequence (NoL…
    Expression
    Mammalian
    Mutation
    Promoter
    RPBSA and TRE promoter
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170152-plasmid-map-sequence-id-335808

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  16. Target-AID (pRZ762)

    Plasmid
    #131300
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    131300-plasmid-map-sequence-id-254651

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  17. pCBh-T7-SpG-P2A-mTagBFP2(RAS3583)

    Plasmid
    #223110
    Purpose
    pCBh Human expression plasmid for SpG with with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpG-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223110-plasmid-map-sequence-id-447633

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  18. Spike Display_Part 1-5 DO

    Plasmid
    #172726
    Purpose
    Encodes a sfGFP dropout expression cassette in place of Parts 1-5 to be used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    172726-plasmid-map-sequence-id-335212

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  20. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid
    #170977
    Purpose
    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.
    Depositor
    Miki Ebisuya
    Article
    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:
    Insert
    CShroom3 (Shroom3 Mouse)
    Use
    Gateway cloning
    Tags
    SspB and mCherry
    Expression
    Mutation
    Isoform X2, deleted aminoacids 1 - 1388
    Promoter
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170977-plasmid-map-sequence-id-336791

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Showing: 6101 - 6120 of 6862 results