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pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

Plasmid

#159838

Purpose

Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

Expression

Worm

Mutation

Not applicable

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMtb MCM

Plasmid

#138296

Purpose

Expression of Mtb MCM small and large subunit

Depositor

Ruma Banerjee

Article

Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

Inserts

mutA
mutB

Tags

His tag and TEV cleavage site

Expression

Bacterial

Mutation

inserted TEV cleavage site between His-tag and m…

Promoter

T7

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Dd-VN

Plasmid

#135517

Purpose

Mammalian expression of VN-fused and myc-tagged H2-Dd

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

C121S

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Ld-VN

Plasmid

#135520

Purpose

Mammalian expression of VN-fused and myc-tagged H2-Ld

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Ld

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-CenpB 1-166

Plasmid

#108474

Purpose

expression of vsv-CenpB 1-166

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB 1-166 AA (CENPB Human)

Tags

VSV

Expression

Mammalian

Mutation

encodes only the DNA-binding domain of CENP-B

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-Mis12-dCEN-Incenp

Plasmid

#108477

Purpose

expression of vsv-Mis12-dCEN-INCENP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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B3GALT5_pCSdest

Plasmid

#53941

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

B3GALT5 (B3GALT5 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
FAM3B_pCSdest

Plasmid

#53933

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

FAM3B (FAM3B Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
ITGB2_pCSdest

Plasmid

#53892

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ITGB2 (ITGB2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
C21orf63_pCSdest

Plasmid

#53857

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

EVA1C (EVA1C Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits