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R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)

Plasmid

#159687

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)

Depositor

Dominic Esposito

Article

Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

Insert

His6-MBP-tev-Hs.RAF1(52-192) (RAF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-PIP5K3

Plasmid

#23806

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIP5K3 (PIKFYVE Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STK38L

Plasmid

#23810

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK38L (STK38L Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv1-sgAAVS1

Plasmid

#83906

Purpose

stable knockout

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

adeno-associated virus integration site 1 (AAVS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRPF4B

Plasmid

#23465

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRPF4B (PRPF4B Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CKM

Plasmid

#23866

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CKM (CKM Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-shRFP

Plasmid

#125778

Purpose

constitutive expression of a short-hairpin RNA targeting RFP

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shRFP

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-SGK

Plasmid

#23708

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SGK (SGK1 Human)

Use

Gateway Cloning

Mutation

F430L

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-NAGK

Plasmid

#23785

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NAGK (NAGK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCIBN-EYFP-hTRF1

Plasmid

#103802

Purpose

BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hTRF1 (CIB1, TERF1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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SHIP2-ΔPS

Plasmid

#214901

Purpose

expression of the truncated variants of the SHIP2 without phosphatase domain

Depositor

Pavel Krejčí

Article

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 delta aa 419-739 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 419-739aa

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-RIOK3

Plasmid

#23537

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIOK3 (RIOK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pD649-HAsp-LILRB3-Fc(DAPA)-AviTag-6xHis

Plasmid

#156848

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LILRB3 (LILRB3 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-TMPRSS2-TwinStrep_Zeo

Plasmid

#192000

Purpose

Lentiviral vector to generate TwinStrep-tagged TMPRSS2 stable expressing cell line

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Tags

TMPRSS2

Expression

Mammalian

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM5-C234S PHGDH

Plasmid

#83902

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

Lentiviral

Expression

Mammalian

Mutation

quikchanged cysteine 234 to serine

Promoter

CMV

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-PHKA2

Plasmid

#23526

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PHKA2 (PHKA2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKCI

Plasmid

#23828

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCI (PRKCI Human)

Use

Gateway Cloning

Mutation

2MM (2 mismatches result in C595R)

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_BCR_JAK2

Plasmid

#205781

Purpose

Express mEGFP-tagged fusion protein, BCR_JAK2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

BCR_JAK2 (BCR, JAK2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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DHC1-AID-Clover donor (Neo)

Plasmid

#158621

Purpose

Donor plasmid for tagging DHC1 with AID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR; Tagging donor

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-RPS6KA4

Plasmid

#23641

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KA4 (RPS6KA4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only