We narrowed to 22,721 results for: TES

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HA-Casp-2L

Plasmid

#157932

Purpose

Over expression of Casp-2L

Depositor

Baoji Xu

Article

Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

Insert

Casp2 (Casp2 Mouse)

Tags

HA and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL_2_tacI

Plasmid

#155174

Purpose

see pDUAL_tacI: exchanged origin of replication (pBR322) and introduced bom site

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL_lacZ

Plasmid

#155169

Purpose

BaSP and CuCBP co-expression: lacZ promoter controls BaSP expression

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBICI_2_medium

Plasmid

#155167

Purpose

see pBICI_medium: exchanged origin of replication (pBR322) and introduced bom site

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBICI_strong

Plasmid

#155165

Purpose

BaSP and CuCBP co-expression: strong RBS controls BaSP expression

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBICI_medium

Plasmid

#155164

Purpose

BaSP and CuCBP co-expression: medium RBS controls BaSP expression

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBICI_weak

Plasmid

#155163

Purpose

BaSP and CuCBP co-expression: weak RBS controls BaSP expression

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-Myosin7B-sgRNA

Plasmid

#154861

Purpose

Lentiviral expression of Cas9 and sgRNA targeting Myosin7B

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

MYO7B sgRNA (MYO7B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.assemble(AB).LC_v1

Plasmid

#133083

Purpose

Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Destination vector

Expression

Bacterial

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.assemble(AB).HC_v1

Plasmid

#133084

Purpose

Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Destination vector

Expression

Bacterial

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K30A

Plasmid

#105694

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K30A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 30 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K31A

Plasmid

#105695

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K31A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 31 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K30A K31A

Plasmid

#105696

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K30/31A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 30 and 31 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_C21A

Plasmid

#105697

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a C21A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Cysteine 21 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K20A

Plasmid

#105698

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K20A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 20 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_S6A

Plasmid

#105699

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a S6A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Serine 6 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PTEN-3'UTR

Plasmid

#97204

Purpose

Expresses PTEN 3'UTR in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Article

Kerwin et al Elife. 2020 Apr 21;9. pii: 51019. doi: 1

Insert

PTEN 3' UTR (PTEN Human)

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pBMN-AS-FOXA1

Plasmid

#139304

Purpose

Knocking down of human FOXA1 through shRNA and amiRNA targeting FOXA1

Depositor

Claes Wadelius

Article

Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

Insert

shRNA and amiRNA against FOXA1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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Y365A or A365Y inducible rescue

Plasmid

#131323

Purpose

gRNA to generate i-WT-r or i-MT-r systems

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

Y365A or A365Y inducible rescue

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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HoxC-downstream-5xTetO Donor

Plasmid

#131341

Purpose

Donor template to insert 5xTet operator sequence into downstream of HoxC cluster.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

HoxC-downstream-5xTetO Donor

Use

CRISPR and Mouse Targeting

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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5xtetO HoxC 3'

Plasmid

#131340

Purpose

gRNA to insert 5xTet operator sequence into downstream of HoxC cluster.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

HoxC-downstream-gRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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MTF2_KO_ex11_gRNA-4

Plasmid

#131334

Purpose

gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

MTF2_KO_ex11_gRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
LMX1B-gRNA-N-del

Plasmid

#131333

Purpose

gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

LMX1B-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HoxD del ds1

Plasmid

#131338

Purpose

gRNA to delete a nucleation site near HoxD. Use with HoxD del us1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EVX2-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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SKOR1-gRNA-C-del

Plasmid

#131332

Purpose

gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-N-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

SKOR1-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SKOR1-gRNA-N-del

Plasmid

#131331

Purpose

gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-C-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

SKOR1-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HoxD del control ds

Plasmid

#131336

Purpose

gRNA to delete control region near HoxD. Use with HoxD del control us

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

CTRL-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
HoxD del us1

Plasmid

#131337

Purpose

gRNA to delete a nucleation site near HoxD. Use with HoxD del ds1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EVX2-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HoxD del control us

Plasmid

#131335

Purpose

gRNA to delete control region near HoxD. Use with HoxD del control ds

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

CTRL-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EZH2-Promoter-gRNA

Plasmid

#131329

Purpose

gRNA to generate endogenously tagged -tetR-EZH2

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EZH2-Promoter-gRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge