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pDONR223_DCAF8_p.G588C

Plasmid

#82792

Purpose

Gateway Donor vector containing DCAF8, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DCAF8 (DCAF8 Human)

Use

Gateway entry vector

Mutation

G588C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IRF4_p.K123R

Plasmid

#81427

Purpose

Gateway Donor vector containing IRF4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRF4 (IRF4 Human)

Use

Gateway entry vector

Mutation

K123R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.1 K+ channel pS603 [L61/14R]

Plasmid

#140063

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ channel pS603 (Mouse). Derived from hybridoma L61/14.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv2.1 K+ channel pS603 (Rat) recombinant mouse monoclonal antibody (Kcnb1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_TP53_p.I195F

Plasmid

#81395

Purpose

Gateway Donor vector containing TP53 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TP53 (TP53 Human)

Use

Gateway entry vector

Mutation

P72R; I195F; P278A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EDNRB_p.F292L

Plasmid

#81402

Purpose

Gateway Donor vector containing EDNRB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EDNRB (EDNRB Human)

Use

Gateway entry vector

Mutation

F292L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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ABL1 gRNA (BRDN0001145259)

Plasmid

#77734

Purpose

3rd generation lentiviral gRNA plasmid targeting human ABL1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ABL1 (ABL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-V765G

Plasmid

#116315

Purpose

Lentiviral expression of EGFR V765G

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

V765G

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-V843I

Plasmid

#116317

Purpose

Lentiviral expression of EGFR V843I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

V843I

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_ACVR2A_p.A381V

Plasmid

#81476

Purpose

Gateway Donor vector containing ACVR2A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ACVR2A (ACVR2A Human)

Use

Gateway entry vector

Mutation

A381V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-P546S

Plasmid

#116282

Purpose

Lentiviral expression of EGFR P546S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

P546S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 5A

Plasmid

#60651

Purpose

Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

mutated five serines to alanines

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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sgEGFR(B)

Plasmid

#85565

Purpose

Expresses sgRNA targeting human EGFR around codon T790

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

epidermal growth factor receptor (EGFR Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
TTN gRNA (BRDN0001145502)

Plasmid

#76851

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTN (TTN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTN gRNA (BRDN0001148405)

Plasmid

#76852

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTN (TTN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTN gRNA (BRDN0001146399)

Plasmid

#76853

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTN (TTN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTN gRNA (BRDN0001148106)

Plasmid

#76854

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTN (TTN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-P596S

Plasmid

#116285

Purpose

Lentiviral expression of EGFR P596S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

P596S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-NSD3 [N348/82R]

Plasmid

#114485

Purpose

Mammalian Expression Plasmid of anti-NSD3 (Human). Derived from hybridoma N348/82.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-NSD3 (Homo sapiens) recombinant mouse monoclonal antibody (NSD3 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_ILKAP_p.S61Y

Plasmid

#81376

Purpose

Gateway Donor vector containing ILKAP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ILKAP (ILKAP Human)

Use

Gateway entry vector

Mutation

S61Y

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.R470H

Plasmid

#81621

Purpose

Gateway Donor vector containing KEAP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Mutation

R470H; C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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