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pBOB-Mrap2-P2A-Mc4r-HA

Plasmid

#245991

Purpose

Stimultaneously express HA C-terminal tagged mouse MC4R and untagged MRAP2 protein using a P2A linker, transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Mrap2 and Mc4r (Mrap2, Mc4r Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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MIGR1-Cdc42-1

Plasmid

#179517

Purpose

Retroviral expression of Cdc42 splice variant 1

Depositor

Bo Porse

Article

Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

Insert

Cdc42 splice variant 1 cDNA (CDC42 Human)

Use

Retroviral

Tags

IRES-EGFP

Promoter

LTR

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_CSNK1A1_WT

Plasmid

#82106

Purpose

Gateway Donor vector containing CSNK1A1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Gateway Cloning

Mutation

Q231K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLX313-TP53-P278A

Plasmid

#118015

Purpose

Constitutive expression of mutant human Tumor Protein p53 (TP53)

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

TP53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

p.Pro278Ala

Promoter

EF1alpha

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-TP53-2

Plasmid

#121918

Purpose

Encodes sgRNA targeting exon 9 of TP53

Depositor

William Hahn

Article

Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

Insert

TP53 (TP53 Human)

Use

CRISPR

Promoter

U6

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.1 K+ channel (External) [K39/25R]

Plasmid

#128620

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (External) (Human). Derived from hybridoma K39/25.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv2.1 K+ Channel (External) (Human) recombinant mouse monoclonal antibody (KCNB1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR2-N549K

Plasmid

#116380

Purpose

Lentiviral expression of FGFR2 N549K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR2 (FGFR2 Human)

Use

Lentiviral

Mutation

N549K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry

Plasmid

#172627

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-HCN4 [N114/10.1R]

Plasmid

#114506

Purpose

Mammalian Expression Plasmid of anti-HCN4 (Human). Derived from hybridoma N114/10.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-HCN4 (Homo sapiens) recombinant mouse monoclonal antibody (HCN4 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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MIGR1-Cdc42-2

Plasmid

#179518

Purpose

Retroviral expression of Cdc42 splice variant 2

Depositor

Bo Porse

Article

Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

Insert

Cdc42 splice variant 2 cDNA (CDC42 Human)

Use

Retroviral

Tags

IRES-EGFP

Promoter

LTR

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF2159

Plasmid

#143347

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

BCL11B (BCL11B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-TRPML3/Mucolipin-3 [N268/19R]

Plasmid

#114477

Purpose

Mammalian Expression Plasmid of anti-TRPML3/Mucolipin-3 (Human). Derived from hybridoma N268/19.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TRPML3/Mucolipin-3 (Homo sapiens) recombinant mouse monoclonal antibody (MCOLN3 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-IFNG-Fc(DAPA)-AviTag-6xHis

Plasmid

#156535

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IFNG (IFNG Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGH020 sgp53

Plasmid

#227947

Purpose

sgRNA targeting p53, GFP, neomycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

Depositor

Laura Attardi

Article

Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

Insert

Tp53 (Trp53 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-H773_V774insNPH

Plasmid

#116262

Purpose

Lentiviral expression of EGFR H773_V774insNPH

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

H773_V774insNPH

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CDKN1B-Puro

Plasmid

#172611

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDKN1B (CDKN1B Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ERBB2_PPP1R1B

Plasmid

#205813

Purpose

Express mEGFP-tagged fusion protein, ERBB2_PPP1R1B from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ERBB2_PPP1R1B (ERBB2, PPP1R1B Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.PAC

Plasmid

#57828

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

Plasmid

#211760

Purpose

Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

Depositor

Glenn Yiu

Article

Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

Insert

gRNA1 and gRNA2 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-CIB1-mCerulean-Rab11

Plasmid

#140574

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Inserts

CIB1 (CIB1 Mustard Weed)
Rab11A (RAB11A Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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