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HUHe variant click editor (CE1) - pCMV-T7-CoCV-nCas9-EcKlenow (JO1262)

Plasmid

#217786

Purpose

Variant CE1 construct with CoCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

CoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-FiCV-nCas9-EcKlenow (JO1263)

Plasmid

#217787

Purpose

Variant CE1 construct with FiCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

FiCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HUHe variant click editor (CE1) - pCMV-T7-GuCV-nCas9-EcKlenow (JO1279)

Plasmid

#217788

Purpose

Variant CE1 construct with GuCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

GuCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HUHe variant click editor (CE1) - pCMV-T7-PCV1-nCas9-EcKlenow (JO1315)

Plasmid

#217789

Purpose

Variant CE1 construct with PCV1 HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV1-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT350 Lenti pEF-dCas9-3XFLAG-MBDa-T2A-tagBFP-P2A-Blast

Plasmid

#187347

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-MBDa-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT351 Lenti pEF-dCas9-3XFLAG-P66_CC-T2A-tagBFP-P2A-Blast

Plasmid

#187348

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-P66_CC-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT256 Lenti pEF-dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast

Plasmid

#187332

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT257 Lenti pEF-dCas9-3XFLAG-Z+F-T2A-tagBFP-P2A-Blast

Plasmid

#187333

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-Z+F+N-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT329 Lenti pEF-rTetR(SE-G72P)-3XFLAG-N+Z+F-T2A-mCherry-BSD-WPRE

Plasmid

#187339

Purpose

rTetR effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

rTetR(SE-G72P)-3XFLAG-N+Z+F-T2A-mCherry-BSD-WPRE

Use

Lentiviral and Synthetic Biology

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT252 Lenti pEF-dCas9-3XFLAG-ZN473_TAD-T2A-tagBFP-P2A-Blast

Plasmid

#187328

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-ZN473_TAD-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT253 Lenti pEF-dCas9-3XFLAG-FOXO3_TAD-T2A-tagBFP-P2A-Blast

Plasmid

#187329

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-FOXO3_TAD-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT254 Lenti pEF-dCas9-3XFLAG-NCOA3_TAD-T2A-tagBFP-P2A-Blast

Plasmid

#187330

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-NCOA3_TAD-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT255 Lenti pEF-dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast

Plasmid

#187331

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT331 Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast

Plasmid

#195538

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT360 AAV-Rapamycin Inducible-noActivator Domain -Citrine

Plasmid

#202058

Purpose

Inducible AAV with reporter gene and cloning site to add activator domains

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-noActivator Domain -Citrine

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITRs intact

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304-BCL-XL-499R

Plasmid

#203574

Purpose

Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499) in mammalian cells.

Depositor

Abhishek Chakraborty

Article

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5- tagged

Expression

Mammalian

Mutation

silent point mutations introduced to make it resi…

Promoter

CMV

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_023d-sgCiBIRC6-1

Plasmid

#202447

Purpose

Expression of a CRISPRi guide targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC-hU6-shBACH1_BFP

Plasmid

#199217

Purpose

Expression plasmid with shRNAmiR targeting BACH1, with a BFP reporter on the backbone.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

shBACH1 (BACH1 Synthetic)

Use

RNAi

Tags

Expression

Mutation

Promoter

human U6

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSITEP-shBIRC6-2(3)-C911

Plasmid

#202452

Purpose

a seed-matched control for pRSITEP-puro-shBIRC6-2(3)

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSITEP-shBIRC6-2(3)

Plasmid

#202451

Purpose

DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003-sgBIRC6-1

Plasmid

#202437

Purpose

Expression of a guide RNA targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_016-sgHRI

Plasmid

#202443

Purpose

Expression of a guide RNA targeting HRI

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

HRI gRNA (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSITEP-shBIRC6-2

Plasmid

#202449

Purpose

DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSITEP-shBIRC6-2-C911

Plasmid

#202450

Purpose

a seed-matched control for pRSITEP-puro-shBIRC6-2

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC_hU6-shRKIP_BFP

Plasmid

#199216

Purpose

Expression plasmid with shRNAmiR targeting RKIP (PEBP1), with a BFP reporter on the backbone.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

shBACH1 (BACH1 Synthetic)

Use

RNAi

Tags

Expression

Mutation

Promoter

human U6

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_023d-sgCiBIRC6-5

Plasmid

#202448

Purpose

Expression of a CRISPRi guide targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003-sgBIRC6-4

Plasmid

#202438

Purpose

Expression of a guide RNA targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003-sgBIRC6-5

Plasmid

#202439

Purpose

Expression of a guide RNA targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-KIR3DL3.1-COMP5AP-AviTag-9xHis

Plasmid

#157299

Purpose

Mammalian expression of cell-surface protein extracellular domain (isoform 1) fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR3DL3 (KIR3DL3 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-KIR3DL3.2-COMP5AP-AviTag-9xHis

Plasmid

#157300

Purpose

Mammalian expression of cell-surface protein extracellular domain (isoform 2) fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR3DL3 (KIR3DL3 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge