We narrowed to 10,155 results for: ADA

1. pEGFP-N1_MeCP2(R306C)

   Plasmid

   #110188

   Purpose

   Expression of mouse MeCP2 (R306C) in mammalian cells

   Depositor

   Adrian Bird

   Article

   Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

   Insert

   MeCP2_e2 FL [R306C] (mouse cDNA) (Mecp2 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Arg 306 Cys

   Promoter

   CMV

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

   Plasmid

   #163572

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-NG-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNOC_hfnCas12a_NR1_NR2

   Plasmid

   #176252

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by full

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-NSD3-short

   Plasmid

   #72552

   Purpose

   expresses 3*FLAG tagged human NSD3-short

   Depositor

   Christopher Vakoc

   Article

   Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

   Insert

   NSD3-short (NSD3 Human)
   

   Use

   Retroviral

   Tags

   3*FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   LTR

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mCherry-FKBP-GAP(ARHGAP29)

   Plasmid

   #222632

   Purpose

   Rho inactivation using rapamycin

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   mCherry-FKBP-GAP(ARHGAP29) (ARHGAP29 Human)
   

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCold I-Hero11

   Plasmid

   #187928

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero11

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero11 (C19orf53 Human)
   

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA-gp78-FLAG RING mutant C356G H361A

   Plasmid

   #61751

   Purpose

   express C-terminally FLAG tagged human gp78

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   gp78 (AMFR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   C356G H361A; P179L, F262L, Y315C, and D346G (ple…

   Promoter

   CMV

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

8. p2R3a-3xVenusYFP-OcsT

   Plasmid

   #71271

   Purpose

   Entry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   3 times VenusYFP
   

   Use

   Gateway

   Tags

   octaline synthase terminator

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-GRAB_CCK1.0

   Plasmid

   #208673

   Purpose

   Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3

    Plasmid

    #170161

    Purpose

    Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME-P2A-H2B-mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

    Plasmid

    #101062

    Purpose

    AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Insert

    CaMPARI2-H396K
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-FCHO1

    Plasmid

    #196910

    Purpose

    visualization of clathrin-coaed vesicle formation in living mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

    Insert

    FCHO1 (FCHO1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1_MeCP2(deltaNC)

    Plasmid

    #110190

    Purpose

    Expression of mouse MeCP2 (deltaNC) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 delta NC (mouse cDNA) - EGFP (Mecp2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta N and C termini

    Promoter

    CMV

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid

    #141236

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    NES-jRGECO1a
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    6xHIS tag and nuclear export signal

    Promoter

    human elongation factor-1 alpha (EF-1 alpha)

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGPN-ACE2

    Plasmid

    #187483

    Purpose

    Expression vector - ACE2

    Depositor

    Lewis Cantley

    Article

    Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Promoter

    EFS

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Raichu-OsRac1 CA

    Plasmid

    #133264

    Purpose

    Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(G19V) (LOC4325879 Oryza sativa)
    

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 G19V

    Promoter

    Ubi

    Available Since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Raichu-OsRac1 DN

    Plasmid

    #133263

    Purpose

    Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(T24N) (LOC4325879 Oryza sativa)
    

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 T24N

    Promoter

    Ubi

    Available Since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPR v2-sgAMPK alpha2 clone1

    Plasmid

    #162122

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha2

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha2 (PRKAA2 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid

    #131312

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-cT3G-cERP2-TSC22D3

    Plasmid

    #192916

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of TSC22D3

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    TSC22D3 (TSC22D3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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