We narrowed to 22,242 results for: NOL

1. pA5c-tesA-vhb50-8fadR

   Plasmid

   #86601

   Purpose

   expression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoter

   Depositor

   Yinjie Tang

   Article

   Liu et al Biotechnol Bioeng. 2016 Aug 6. doi: 10.1

   Inserts

   tesA
   Vhb50
   fadR
   

   Expression

   Bacterial

   Promoter

   LacUV5 and pBAD

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCARS1

   Plasmid

   #174868

   Purpose

   Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1

   Depositor

   Zengyi Shao

   Article

   Lopez et al Appl Microbiol Biotechnol. 2021 Aug 6. p

   Insert

   ARS1
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. ND4.2-Right DdCBE-G1397-C-wildtype

   Plasmid

   #179687

   Purpose

   Expression of base editor in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C )_1xUGI_SOD2 3'UTR
   

   Expression

   Mammalian

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJIT163-AFID-3

   Plasmid

   #167359

   Purpose

   For inducing predictable deletions in protoplasts and wheat plants

   Depositor

   Caixia Gao

   Article

   Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

   Insert

   A3A-Cas9-UDG-T2A-AP lyase
   

   Expression

   Plant

   Mutation

   WT

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19/mChe-Cs

   Plasmid

   #183171

   Purpose

   Construct for transient expression of cytoskeleton-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   mCherry-Cs
   

   Tags

   363 aa of A. thaliana FIMBRIN 1 (AtFIM1)

   Expression

   Plant

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CJ8421_04975 Rptr2 and CJ8421_04975 mRNA

   Plasmid

   #194210

   Purpose

   Expresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2

   Depositor

   Chase Beisel

   Article

   Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

   Insert

   CJ8421_04975 mRNA and CJ8421_04975 Rptr2
   

   Expression

   Bacterial

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC-H1-gRNA

   Plasmid

   #61089

   Purpose

   CRISPR/Cas9 system gRNA cloning

   Depositor

   Shenglin Huang

   Article

   Zheng et al Biotechniques. 2014 Sep 1;57(3):115-24.

   Insert

   H1 promoter; gRNA sequences
   

   Use

   CRISPR; /cas9 grna cloning vector

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD2529-CAG-Flag-mouse TGFb1

   Plasmid

   #180260

   Purpose

   Mouse full length TGFβ1 expression

   Depositor

   Timothy Springer

   Article

   Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

   Insert

   TGFβ1 (Tgfb1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. c3GIC9

   Plasmid

   #62191

   Purpose

   col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

   Depositor

   Lukas Dow

   Article

   Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

   Insert

   humanized S. Pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Promoter

   TRE3G

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. SB_loop

    Plasmid

    #110103

    Purpose

    pBXPHM3 with scaffold Sybody of the loop library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the loop library
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTH1-SaSrtA

    Plasmid

    #64973

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    T7 promoter

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMM131

    Plasmid

    #127214

    Purpose

    BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, AtSTM, sgRNA
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19/CF-mChe

    Plasmid

    #183166

    Purpose

    Construct for transient expression of cytoplasmic foci-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    CF-mCherry
    

    Tags

    AtTZF1, A. thaliana TANDEM ZINC FINGER PROTEIN 1

    Expression

    Plant

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. GB4080

    Plasmid

    #215249

    Purpose

    Module for the constitutive expression of EGFP, p19 and the N. nambi luminescence pathway genes fused to a SF.

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    SF-EGFP-p19-LUZ-H3H-HISPS-CPH
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH-A3A-PBE

    Plasmid

    #119774

    Purpose

    Conversion of C to T in rice plants transformation

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. VPR_dCas9

    Plasmid

    #134601

    Purpose

    Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker

    Depositor

    Lasse Ebdrup Pedersen

    Article

    Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-5

    Insert

    VPR-dCas9-2A-ZsGreen-DR
    

    Tags

    VPR and ZsGreen1-DR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. tracrRNA(scr)-CJ8421_04975 mRNA

    Plasmid

    #199718

    Purpose

    Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 mRNA and scrambled tracrRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC1430

    Plasmid

    #127981

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 3xF-GAR1

    Plasmid

    #126873

    Purpose

    Expresses 3xFlag GAR1

    Depositor

    Kathleen Collins

    Article

    Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

    Insert

    GAR1 (GAR1 Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBT100.106b

    Plasmid

    #122601

    Purpose

    clone APOBEC1 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    APOBEC1
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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