We narrowed to 9,470 results for: pho;

1. pGEX-GST-BcPC-PLC

   Plasmid

   #224255

   Purpose

   Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

   Depositor

   Chiaki Murakami

   Article

   Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

   Insert

   Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
   

   Use

   Tags

   GST and HRV-3C (LEVLFQGP) site

   Expression

   Bacterial

   Mutation

   The gene of Bacillus cereus ATCC 14579 was used f…

   Promoter

   lac promoter

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-Ec.coaBC (pCLK1210)

   Plasmid

   #50448

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

   Depositor

   Erick Strauss, Tadhg Begley

   Article

   Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

   Insert

   PPCS PPCDC
   

   Use

   Tags

   6XHIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. EcPhnD90.L1∆∆AD.L297RL301R.pRSET

   Plasmid

   #33376

   Purpose

   a single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFP

   Depositor

   Loren Looger

   Article

   Alicea et al J Mol Biol. 2011 Oct 12.

   Insert

   phnD based Phosphonate Biosensor (phnD E. coli)
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from GCaMP2 inserted in place of…

   Promoter

   T7

   Available Since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. mRFP-FKBP-INPP5E(D556A)

   Plasmid

   #183678

   Purpose

   Recruitable human type IV 5-phosphatase enzyme (catalytically inactive)

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   INPP5E (INPP5E Human)
   

   Use

   Tags

   mRFP-FKBP12(1-108)

   Expression

   Mammalian

   Mutation

   C641A, D556A

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. tdTomato-anti-AlfaTag nanobody

   Plasmid

   #171819

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   tdTomato-anti-AlfaTag nanobody
   

   Use

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SBFP2-anti-AlfaTag nanobody

   Plasmid

   #171826

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   SBFP2-anti-AlfaTag nanobody
   

   Use

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. cfSGFP2-anti-AlfaTag nanobody

   Plasmid

   #171818

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   cfSGFP2-anti-AlfaTag nanobody
   

   Use

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-PLA2G15-6xHis WT

   Plasmid

   #213603

   Purpose

   Expresses PLA2G15-6xHis in mammalian cells

   Depositor

   Monther Abu-Remaileh

   Article

   Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

   Insert

   Phospholipase A2 Group XV (PLA2G15 Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p15aC-4D1D-5

   Plasmid

   #107866

   Purpose

   Bacterial expression of PIS, PI4K and PI4P5K

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Inserts

   PIS
   phosphatidylinositol 4-phosphate 5-kinase
   phosphatidylinositol 4-kinase β
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   Promoter

   proD and proD (in operon)

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNAS1b split mCherry 14-3-3β

    Plasmid

    #111880

    Purpose

    Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Use

    Tags

    Split mCherry system: 14-3-3β fused to C-terminal…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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