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pPB-cT3G-cERP2-ESX1

Plasmid

#222563

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ESX1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

ESX1 (ESX1 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-Dm4E-T_9-44-F41DW42D-GB1_AA

Plasmid

#148256

Purpose

Bacterial Expression of Dm4E-T_9-44-F41DW42D

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dm4E-T_9-44-F41DW42D (4E-T Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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RVD-AC9

Plasmid

#173256

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Insert

NI-HD dimer RVD at Position9

Use

Unspecified

Mutation

no

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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FnRPR9-10

Plasmid

#182568

Purpose

Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 9-10

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1374A, P1376A, R1379A

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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p-preSufI(IAC)

Plasmid

#168516

Purpose

Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

pre-SufI(IAC)

Tags

6xHisC tag and HSV tag

Expression

Bacterial

Mutation

C17I and C295A

Promoter

T7

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET3a-FruK

Plasmid

#186256

Purpose

Overexpression of FruK for purification

Depositor

Liskin Swint-Kruse, Emile Van Schaftingen

Article

Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

Insert

1-phosphofructokinase (fruK E. coli)

Expression

Bacterial

Promoter

T7

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-3xHA-Syn-smFP-flag

Plasmid

#182563

Purpose

For mouse Nav1.6 knockin with 3xHA at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xHA donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-HaloTag-V5-EF1-sfGFP

Plasmid

#182565

Purpose

For mouse Nav1.6 knockin with HaloTag-V5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and HaloTag-V5 donor DNA
sfGFP

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-3xV5-EF1-smFP-flag

Plasmid

#182562

Purpose

For mouse Nav1.6 knockin with 3xV5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMRBad-Z-C-wtBlc

Plasmid

#168256

Purpose

C-fragment. This plasmid can be co-transformed with either Plasmid 168258: pET11a-Z-N-wtBlc or Plasmid 168473: pET11a-Z-N-DiB2 to obtain wtBlc‐split-Zip or DiB2‐split-Zip protein, correspondingly

Depositor

Jens Meiler

Article

Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:

Insert

Leucine Zipper + C-fragment of wtBlc

Expression

Bacterial

Promoter

araBAD

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:ER-S11-GFP1–9

Plasmid

#125666

Purpose

Binary vector for the inducible expression of ER-targeted GFP1–9 fragment that can be used to detect a protein tagged with GFP10

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

ER-S11-GFP1–9

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*R47W)

Plasmid

#125659

Purpose

pCMV-ABEmax(TadA E59A, TadA*R47W) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* R47W)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*R47M)

Plasmid

#125660

Purpose

pCMV-ABEmax(TadA E59A, TadA*R47M) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* R47M)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*D108M)

Plasmid

#125656

Purpose

pCMV-ABEmax(TadA E59A, TadA*D108M) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* D108M)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*R47F)

Plasmid

#125658

Purpose

pCMV-ABEmax(TadA E59A, TadA*R47F) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* D108F)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*D108W)

Plasmid

#125653

Purpose

pCMV-ABEmax(TadA E59A, TadA*D108W) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* D108W)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*D108F)

Plasmid

#125654

Purpose

pCMV-ABEmax(TadA E59A, TadA*D108F) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* D108F)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*V106M)

Plasmid

#125650

Purpose

pCMV-ABEmax(TadA E59A, TadA*V106M) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* V106M)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*V106F)

Plasmid

#125652

Purpose

pCMV-ABEmax(TadA E59A, TadA*V106F) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* V106F)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV myc DAF-12(P687S)

Plasmid

#32567

Depositor

Al Fisher

Article

Hochbaum et al PLoS Genet. 2011 Jul;7(7):e1002179. Epub

Insert

DAF-12(P687S) (daf-12 Nematode)

Tags

myc epitodpe

Expression

Mammalian

Mutation

Proline 687 to Serine

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

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